Cunningham R K, Thacore H R, Zhou P, Nakeeb S, Zaleski M B
Department of Microbiology, State University of New York, Buffalo 14214.
Int Arch Allergy Immunol. 1994;103(1):16-22. doi: 10.1159/000236600.
Lymphoproliferative disease was elicited in C57BL/6KH and (BALB/c x C57BL/6)F1 hybrids by a single intraperitoneal injection of 10(5) FFU of LP-BM5 virus preparation. The disease could reproducibly be transferred by a single intravenous transfusion of 0.2 ml of whole blood as well as 0.1 ml of blood cells, plasma or serum from the infected animals. F1 hybrids displayed a delayed development of the disease when an acellular virus preparation was administered, but they were fully susceptible to the disease when syngeneic blood from infected F1 donors was transfused. Blood from donors in the prodromal stage was as effective in transmission of the disease as blood from donors with fully developed disease. This indicated that in murine lymphoproliferative disease viremia develops very early in the course of the disease. It seems that using the blood transfusion one could develop a reliable semiquantitative assay for the infectiveness of the animals suffering from LP-MB5-induced lymphoproliferative disease.
通过腹腔内单次注射10(5) FFU的LP - BM5病毒制剂，在C57BL/6KH和(BALB/c×C57BL/6)F1杂交小鼠中引发了淋巴增殖性疾病。该疾病可以通过单次静脉输注0.2 ml全血以及0.1 ml来自感染动物的血细胞、血浆或血清而可重复地传播。当给予无细胞病毒制剂时，F1杂交小鼠表现出疾病发展延迟，但当输注来自感染F1供体的同基因血液时，它们对该疾病完全易感。前驱期供体的血液在疾病传播方面与疾病完全发展的供体的血液一样有效。这表明在小鼠淋巴增殖性疾病中，病毒血症在疾病过程中很早就会出现。似乎通过输血可以开发一种可靠的半定量测定方法，用于检测患有LP - MB5诱导的淋巴增殖性疾病的动物的感染性。
