Rep M, Grivell L A
Section for Molecular Biology, Department of Molecular Cell Biology, University of Amsterdam, Kruislaan 318, 1098 SM Amsterdam, The Netherlands.
Curr Genet. 1996 Nov;30(5):367-80. doi: 10.1007/s002940050145.
It has been known for a long time that mitochondria contain their own protein-degradation systems. Only recently, however, have genes for mitochondrial proteases been identified and the powerful techniques of molecular biology been applied to gain insight into the role of protein degradation in mitochondrial biogenesis. It is now clear that the mitochondrial proteases that are involved in the initial stages of degradation are similar to prokaryotic ATP-dependent proteases, and that a division of labour exists between soluble and membrane-bound systems. These systems are essential for the biogenesis of fully functional mitochondria. Their natural targets are currently being identified, and their co-operation with chaperones and possible dual functions as chaperones/proteases are being investigated.
长期以来，人们一直知道线粒体含有自身的蛋白质降解系统。然而，直到最近，线粒体蛋白酶的基因才得以鉴定，分子生物学的强大技术也才被应用于深入了解蛋白质降解在线粒体生物发生中的作用。现在很清楚，参与降解初始阶段的线粒体蛋白酶与原核生物ATP依赖性蛋白酶相似，并且在可溶性和膜结合系统之间存在分工。这些系统对于功能完备的线粒体的生物发生至关重要。它们的天然靶标目前正在被鉴定，并且它们与伴侣蛋白的合作以及作为伴侣蛋白/蛋白酶的可能双重功能也正在被研究。
