Kakkar R, Bains J S, Sharma S P
Saskatoon Cancer Centre-Research Unit, University of Saskatchewan, Canada.
Gerontology. 1996;42(6):312-21. doi: 10.1159/000213809.
Zaprionus paravittiger fed with vitamin E supplemented diet (1, 5 and 10 micrograms/ml) showed an increase in median and maximum life spans. Further increase in concentration accelerated the mortality rate. Females exhibited longer life span as compared to males. Malondialdehyde (MDA) content and antioxidant enzymes (catalase and peroxidase) were measured in control and optimum concentration of vitamin E (5 micrograms/ml)-fed flies at various age intervals. MDA content showed an increase with age in control and vitamin E-fed group whereas catalase and proxidase activities showed a decrease with age. The females exhibited lower MDA content and higher activities of catalase and peroxidase as compared to males in control and vitamin E group. Vitamin E feeding caused a significant decrease in MDA and increase in catalase and peroxidase activities. These findings suggest that vitamin E has dose-dependent and sex-specific influence on longevity of Z. paravittiger and support the view that longevity and activity of antioxidant enzymes are positively linked.
用补充了维生素E的饲料（1、5和10微克/毫升）喂养的副带蚤蝇，其平均寿命和最长寿命有所增加。浓度进一步增加会加速死亡率。与雄性相比，雌性的寿命更长。在不同年龄阶段，对对照组和用最佳浓度维生素E（5微克/毫升）喂养的果蝇测定了丙二醛（MDA）含量和抗氧化酶（过氧化氢酶和过氧化物酶）。在对照组和维生素E喂养组中，MDA含量均随年龄增长而增加，而过氧化氢酶和过氧化物酶活性则随年龄增长而降低。在对照组和维生素E组中，与雄性相比，雌性的MDA含量较低，过氧化氢酶和过氧化物酶活性较高。维生素E喂养导致MDA显著降低，过氧化氢酶和过氧化物酶活性增加。这些发现表明，维生素E对副带蚤蝇的寿命具有剂量依赖性和性别特异性影响，并支持了寿命与抗氧化酶活性呈正相关的观点。
