Bains J S, Garg S K, Sharma S P
Department of Pathology, University of Calgary, Alta., Canada.
Gerontology. 1998;44(5):262-6. doi: 10.1159/000022022.
Catalase activity and malondialdehyde (MDA) content were measured in whole body and mitochondrial homogenates of the banana fruit fly, Zaprionus paravittiger, fed on control and butylated hydroxyanisole (BHA, 10 mM) mixed diets. Catalase activity increased during the reproductive period and decreased thereafter with age. However, the MDA content increased with advancing age in both sexes. In general, females exhibited higher catalase activity and lower MDA content as compared to their male counterparts. BHA feeding increased catalase activity significantly during all age intervals in both sexes. Mitochondrial fractions had lower catalase activity and lower MDA content than whole body homogenates. However, the pattern of changes was similar in both homogenates with age as well as on antioxidant feeding. These results suggest that BHA strengthens the defense mechanism of the insects by increasing catalase activity and reducing MDA content which may be responsible for increased longevity of insects.
在以对照饮食和丁基羟基茴香醚（BHA，10 mM）混合饮食喂养的拟 vittiger 果蝇（Zaprionus paravittiger）的全身和线粒体匀浆中，测定了过氧化氢酶活性和丙二醛（MDA）含量。过氧化氢酶活性在生殖期增加，此后随年龄增长而降低。然而，两性的 MDA 含量均随年龄增长而增加。一般来说，与雄性果蝇相比，雌性果蝇表现出更高的过氧化氢酶活性和更低的 MDA 含量。在所有年龄阶段，喂食 BHA 均显著提高了两性的过氧化氢酶活性。线粒体部分的过氧化氢酶活性和 MDA 含量低于全身匀浆。然而，两种匀浆中随年龄以及抗氧化剂喂养的变化模式相似。这些结果表明，BHA 通过增加过氧化氢酶活性和降低 MDA 含量来增强昆虫的防御机制，这可能是昆虫寿命延长的原因。
