Tanaka Y, Shiwa M, Kojima H, Miyazaki H, Kamide Y, Moriyama H
Department of Otorhinolaryngology, Jikei University School of Medicine, Tokyo, Japan.
Laryngoscope. 1998 Apr;108(4 Pt 1):537-42. doi: 10.1097/00005537-199804000-00014.
With the objective of estimating proliferation ability of epidermis of middle ear cholesteatoma, the difference in proliferating cell nuclear antigen (PCNA) staining between the skin of the bone region of the external ear canal (control skin) and cholesteatoma epidermis and the effects on PCNA staining of subepidermal inflammatory cell infiltration of cholesteatoma were immunohistochemically studied using an antibody against PCNA. Transforming growth factor-alpha (TGF-alpha) is known to promote epidermal proliferation based on autocrine mechanism. But it is not clear that cholesteatoma epidermis is actually in the state of hyperproliferation under the effect of this growth factor. To estimate the effect of TGF-alpha on epidermal proliferation ability, the authors compared the location of PCNA and TGF-alpha in the same specimen. Unlike the control skin, not only epidermal basal cell layer and suprabasal cell layer, but also more superior layers were found to have high levels of PCNA staining in the epidermis of cholesteatoma. However, in the same cholesteatoma epidermal tissue, the PCNA staining was varied and the difference was ascribable to subepidermal cell inflammation. It appeared that the proliferation ability was high in regions where subepidermal inflammatory cell infiltration was severe. These differences in microenvironment are inferred to greatly affect proliferation ability of cholesteatoma epidermis.
为评估中耳胆脂瘤表皮的增殖能力，采用抗增殖细胞核抗原（PCNA）抗体，通过免疫组织化学方法研究外耳道骨部皮肤（对照皮肤）与胆脂瘤表皮PCNA染色的差异，以及胆脂瘤表皮下炎性细胞浸润对PCNA染色的影响。已知转化生长因子-α（TGF-α）通过自分泌机制促进表皮增殖。但尚不清楚胆脂瘤表皮在这种生长因子作用下是否实际处于增殖过度状态。为评估TGF-α对表皮增殖能力的影响，作者在同一标本中比较了PCNA和TGF-α的定位。与对照皮肤不同，胆脂瘤表皮不仅基底细胞层和基底上层PCNA染色水平高，而且更高层也有高水平染色。然而，在同一胆脂瘤表皮组织中，PCNA染色存在差异，且差异归因于表皮下细胞炎症。似乎在表皮下炎性细胞浸润严重的区域增殖能力较高。推测这些微环境差异对胆脂瘤表皮的增殖能力有很大影响。
