Kojima H, Tanaka Y, Tanaka T, Miyazaki H, Shiwa M, Kamide Y, Moriyama H
Department of Otorhinolaryngology, Jikei University School of Medicine, Tokyo, Japan.
Arch Otolaryngol Head Neck Surg. 1998 Mar;124(3):261-4. doi: 10.1001/archotol.124.3.261.
To compare the pattern of proliferation and apoptotic cell death in cholesteatoma tissues with that in normal skin.
The cholesteatoma tissue samples were excised from 10 patients during surgery. Normal skin specimens collected from the external ear canal of 6 of the 10 patients were used as controls.
In all cholesteatoma tissue samples, apoptotic cells were not seen in the basal cell layer, but they were observed in the suprabasal, prickle, and granular cell layers. In skin specimens obtained from normal external ear canal skin, in which the suprabasal cell layer was comparatively small, similar kinetics of apoptotic cell death were observed. Immunohistochemical analysis using a monoclonal antibody to proliferation cell nuclear antigen demonstrated the presence of proliferating cells in the basal and suprabasal cell layers of the normal external ear canal skin, whereas in the cholesteatoma tissue samples, large numbers of proliferation cell nuclear antigen-positive cells were also observed in the prickle and granular cell layers.
Proliferation in cholesteatoma epidermal cells is not uncontrolled, as it is in malignant tumors. Our results demonstrate an increase in the rate of proliferation and apoptotic cell death in cholesteatoma epidermis.
比较胆脂瘤组织与正常皮肤中细胞增殖和凋亡性细胞死亡的模式。
手术中从10例患者身上切除胆脂瘤组织样本。从这10例患者中的6例外耳道采集的正常皮肤标本用作对照。
在所有胆脂瘤组织样本中，未见基底细胞层中有凋亡细胞，但在基底上层、棘层和颗粒层中观察到凋亡细胞。在取自正常外耳道皮肤的标本中，基底上层细胞层相对较小，观察到类似的凋亡性细胞死亡动力学。使用增殖细胞核抗原单克隆抗体进行的免疫组织化学分析表明，正常外耳道皮肤的基底和基底上层细胞层中有增殖细胞，而在胆脂瘤组织样本中，在棘层和颗粒层中也观察到大量增殖细胞核抗原阳性细胞。
胆脂瘤表皮细胞的增殖并非如恶性肿瘤那样不受控制。我们的结果表明胆脂瘤表皮中增殖率和凋亡性细胞死亡增加。
