Cherayil J D, Lipsett M N
J Bacteriol. 1977 Sep;131(3):741-4. doi: 10.1128/jb.131.3.741-744.1977.
Until recently, the presence in transfer ribonucleic acid (tRNA) of the hydroxylated cytokinin ribosylzeatin [N6-(4-hydroxy-3-methylbut-2-enyl)adenosine]was thought to be unique to higher plants. This extension of work from several laboratories indicates the presence of 2-methylthioribosylzeatin in the tRNA of the plant-associated bacteria Rhizobium leguminosarum, Agrobacterium tumefaciens, and Corynebacterium fascians, but not in that of Erwinia amylovora. This cytokinin has the cis configuration, as is normally found in the tRNA's of plants. The tRNA thionucleotide patterns in these bacteria are different from those of Escherichia coli, Bacillus subtilis, and Salmonella typhimurium, which contain the unhydroxylated analogs of ribosylzeatin or 2-methylthioribosylzeatin.
直到最近,人们一直认为羟基化细胞分裂素核糖玉米素[N6-(4-羟基-3-甲基丁-2-烯基)腺苷]在转运核糖核酸(tRNA)中的存在是高等植物所特有的。来自几个实验室的这项扩展研究表明,在与植物相关的细菌豆科根瘤菌、根癌农杆菌和 fascians 棒杆菌的 tRNA 中存在 2-甲基硫代核糖玉米素,但在梨火疫病菌的 tRNA 中不存在。这种细胞分裂素具有顺式构型,这在植物的 tRNA 中通常可以发现。这些细菌中的 tRNA 硫核苷酸模式与大肠杆菌、枯草芽孢杆菌和鼠伤寒沙门氏菌不同,后者含有核糖玉米素或 2-甲基硫代核糖玉米素的未羟基化类似物。