恶臭假单胞菌中的肌醇转运系统。
Myo-inositol transport system in Pseudomonas putida.
作者信息
Rber G, Belet M, Deshusses J
出版信息
J Bacteriol. 1977 Sep;131(3):872-5. doi: 10.1128/jb.131.3.872-875.1977.
Abstract
The kinetic features of the myo-inositol transport system in Pseudomonas putida are reported. The system is sensitive to osmotic shock, is not operative in membrane vesicles, and does not involved substrate phosphorylation. Line-weaver-Burk plots indicate the presence of two different systems, whose Kt are 5 micrometer and 0.43 mM and whose V max are 7.9 and 27 nml/mg per min, respectively. Transport activity of glucose-grown cells is very low. myo-Inositol-grown cells lose the high-affinity system upon osmotic shock; concentrated shock fluid possesses myo-inositol-binding activity. The system is very specific for the myo-configuration of the cyclitol.
摘要
本文报道了恶臭假单胞菌中肌醇转运系统的动力学特征。该系统对渗透压休克敏感，在膜泡中不起作用，且不涉及底物磷酸化。Line-weaver-Burk图表明存在两种不同的系统，其Kt分别为5微摩尔和0.43毫摩尔，Vmax分别为7.9和27纳升/毫克每分钟。葡萄糖培养的细胞的转运活性非常低。肌醇培养的细胞在渗透压休克后失去高亲和力系统；浓缩的休克液具有肌醇结合活性。该系统对环醇的肌醇构型具有高度特异性。
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