来自利用碳氢化合物的热带假丝酵母细胞表面的成分，可能与碳氢化合物运输有关。
Component from the cell surface of the hydrocarbon-utilizing yeast Candida tropicalis with possible relation to hydrocarbon transport.
作者信息
Käppeli O, Fiechter A
出版信息
J Bacteriol. 1977 Sep;131(3):917-21. doi: 10.1128/jb.131.3.917-921.1977.
Abstract
A polysaccharide-fatty acid complex was isolated from the cell surface of Candida tropicalis growing on alkanes. This complex was solubilized by Pronase treatment of whole cells. A decrease in alkane-binding affinity was observed after Pronase treatment, resulting in 10 to 12% of the yeast dry cell weight being released as polysaccharide. The isolated polysaccharide contained 2.5% fatty acids. C. tropicalis and Saccharomyces cerevisiae grown with glucose contained only traces of fatty acids in the corresponding polysaccharide fraction. The fatty acids were not removed from the polysaccharide moiety by gel filtration. Extraction of the polysaccharide with chloroform-methanol showed that fatty acids were covalently bound to the polysaccharide. The amphipathic nature of the isolated polysaccharide and the hydrocarbon-induced formation suggest a possible role in alkane metabolism.
摘要
从生长在烷烃上的热带假丝酵母细胞表面分离出一种多糖 - 脂肪酸复合物。通过用链霉蛋白酶处理全细胞可使该复合物溶解。链霉蛋白酶处理后观察到烷烃结合亲和力降低，导致10%至12%的酵母干细胞重量以多糖形式释放。分离出的多糖含有2.5%的脂肪酸。用葡萄糖培养的热带假丝酵母和酿酒酵母在相应的多糖部分仅含有痕量脂肪酸。通过凝胶过滤未从多糖部分去除脂肪酸。用氯仿 - 甲醇提取多糖表明脂肪酸与多糖共价结合。分离出的多糖的两亲性质和碳氢化合物诱导的形成表明其在烷烃代谢中可能发挥作用。
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本文引用的文献
1
Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
J Biol Chem. 1951 Nov;193(1):265-75.
2
[The structure of the yeast cell wall. II. Degradative studies with enzymes].[酵母细胞壁的结构。II. 酶降解研究]
Proc R Soc Lond B Biol Sci. 1958 Dec 17;149(936):425-40. doi: 10.1098/rspb.1958.0085.
3
Hydrocarbon fermentations using Candida lipolytica. II. A model for cell growth kinetics.利用解脂耶氏酵母进行烃类发酵。II. 细胞生长动力学模型。
Biotechnol Bioeng. 1971 Nov;13(6):761-78. doi: 10.1002/bit.260130604.
4
A simple and convenient procedure for the hydrogenation of lipids on the micro- and nanomole scale.一种用于微摩尔和纳摩尔规模脂质氢化的简单便捷方法。
J Lipid Res. 1972 Jan;13(1):146-8.
5
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6
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Adv Enzymol Relat Areas Mol Biol. 1974;40(0):239-70. doi: 10.1002/9780470122853.ch6.
7
The functional role of lipids in hydrocarbon assimilation.脂质在碳氢化合物同化中的功能作用。
Biotechnol Bioeng. 1974 Jul;16(7):965-85. doi: 10.1002/bit.260160709.
8
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