Käppeli O, Fiechter A
J Bacteriol. 1977 Sep;131(3):917-21. doi: 10.1128/jb.131.3.917-921.1977.
A polysaccharide-fatty acid complex was isolated from the cell surface of Candida tropicalis growing on alkanes. This complex was solubilized by Pronase treatment of whole cells. A decrease in alkane-binding affinity was observed after Pronase treatment, resulting in 10 to 12% of the yeast dry cell weight being released as polysaccharide. The isolated polysaccharide contained 2.5% fatty acids. C. tropicalis and Saccharomyces cerevisiae grown with glucose contained only traces of fatty acids in the corresponding polysaccharide fraction. The fatty acids were not removed from the polysaccharide moiety by gel filtration. Extraction of the polysaccharide with chloroform-methanol showed that fatty acids were covalently bound to the polysaccharide. The amphipathic nature of the isolated polysaccharide and the hydrocarbon-induced formation suggest a possible role in alkane metabolism.
从生长在烷烃上的热带假丝酵母细胞表面分离出一种多糖 - 脂肪酸复合物。通过用链霉蛋白酶处理全细胞可使该复合物溶解。链霉蛋白酶处理后观察到烷烃结合亲和力降低，导致10%至12%的酵母干细胞重量以多糖形式释放。分离出的多糖含有2.5%的脂肪酸。用葡萄糖培养的热带假丝酵母和酿酒酵母在相应的多糖部分仅含有痕量脂肪酸。通过凝胶过滤未从多糖部分去除脂肪酸。用氯仿 - 甲醇提取多糖表明脂肪酸与多糖共价结合。分离出的多糖的两亲性质和碳氢化合物诱导的形成表明其在烷烃代谢中可能发挥作用。
