Kaeppeli O, Fiechter A
Biotechnol Bioeng. 1976 Jul;18(7):967-74. doi: 10.1002/bit.260180709.
A method for the measurement of the affinity of the cell surface to hydrocarbons is described. The affinity was basically unaffected by different pH values and temperature as well as by the chain length of the substrate. The contact time required for saturation of the cell surface with substrate was 30 sec. Cells grown on glucose showed a 25% lower adsorption capacity compared to those grown on n-alkane. The glucose grown cells showed also a more distinguished dependence of the amount of adsorbed hydrocarbon on the quality of the emulsion. The interaction between the substrate and cell surface turned out to be an adsorption that did not involve an enzymatic reaction. These results led to the conclusion that a lipopolysaccharide moiety present at the cell surface is responsible for the affinity.
本文描述了一种测量细胞表面对碳氢化合物亲和力的方法。该亲和力基本不受不同pH值、温度以及底物链长的影响。细胞表面被底物饱和所需的接触时间为30秒。在葡萄糖上生长的细胞与在正构烷烃上生长的细胞相比，吸附能力低25%。在葡萄糖上生长的细胞对吸附的碳氢化合物量对乳液质量的依赖性也更明显。结果表明，底物与细胞表面之间的相互作用是一种不涉及酶反应的吸附作用。这些结果得出结论，细胞表面存在的脂多糖部分是造成这种亲和力的原因。
