McNally A J, Pilcher I, Wu R, Salamone S J, Brewington S, King J W, Irving J
Roche Diagnostic Systems, Somerville, New Jersey 08876, USA.
J Anal Toxicol. 1996 Nov-Dec;20(7):537-40. doi: 10.1093/jat/20.7.537.
A study was conducted to compare the clinical sensitivity of the OnLine and EMIT II assays for propoxyphene (PPX) use in human urine. A total of 5138 random clinical samples were evaluated by both OnLine and EMIT II. Samples that were positive for each immunoassay were confirmed for PPX and norpropoxyphene (NPPX) by gas chromatography-mass spectrometry (GC-MS). There were 14 samples that were identified positive by both immunoassays and confirmed positive by GC-MS. An additional six samples were positive by OnLine, negative by EMIT II, and confirmed positive by GC-MS. There was one unconfirmed positive sample identified by each immunoassay, and 5116 samples were identified as negative by both immunoassays. The increased sensitivity by OnLine can be attributed to the cross reactivity of the OnLine antibody, which is higher than the cross reactivity of the EMIT II antibody for NPPX (77% versus 7%, respectively). The high concentrations of NPPX, relative to those of PPX, found in all of the clinical samples suggest that laboratories that currently confirm for PPX should confirm for NPPX in order to obtain a better correlation between immunoassay results and GC-MS confirmations.
开展了一项研究，以比较在线检测法和EMIT II检测法在检测人尿中丙氧芬（PPX）时的临床灵敏度。共有5138份随机临床样本同时采用在线检测法和EMIT II检测法进行评估。每种免疫测定呈阳性的样本通过气相色谱-质谱联用仪（GC-MS）确认是否含有PPX和去甲丙氧芬（NPPX）。有14份样本两种免疫测定均呈阳性且经GC-MS确认为阳性。另外有6份样本在线检测呈阳性，EMIT II检测呈阴性，但经GC-MS确认为阳性。每种免疫测定均鉴定出1份未经确认的阳性样本，5116份样本两种免疫测定均鉴定为阴性。在线检测法灵敏度更高的原因可能是在线检测法抗体的交叉反应性高于EMIT II检测法抗体对NPPX的交叉反应性（分别为77%和7%）。在所有临床样本中发现，相对于PPX而言，NPPX的浓度较高，这表明目前对PPX进行确认的实验室也应对NPPX进行确认，以便使免疫测定结果与GC-MS确认结果之间具有更好的相关性。
