Hatakeyama S, Iwabuchi K, Ato M, Iwabuchi C, Kajino K, Takami K, Katoh M, Ogasawara K, Good R A, Onoé K
Section of Pathology, Hokkaido University, Sapporo, Japan.
Microbiol Immunol. 1996;40(3):223-31. doi: 10.1111/j.1348-0421.1996.tb03338.x.
The c-fgr gene product (Fgr) is a member of the src-family of protein tyrosine kinases. We have established a monoclonal antibody (2H2) which recognizes the unique N-terminal domain of the murine Fgr. In the present study, using immunohistochemical analysis and immune complex kinase assay with the 2H2, we investigated expression of Fgr in various cell populations and tissues in a murine system. In resting conditions, Fgr expression was confined to subsets of a monocyte/macrophage lineage. Thus, Fgr+ cells were detected in paracortical areas and medullas of lymph nodes, but seen only in marginal zones of the spleen and the medulla of the thymus. No Fgr+ macrophage was detected in other tissues, Peyer's patches, brain, heart, lung, liver, pancreas, kidney and peritoneal cavity. However, immune complex kinase assay revealed that, upon stimulation, T and B cells as well as peritoneal macrophages expressed significant levels of Fgr molecules. Transformed cell lines of lymphoid origin, EL-4 and LK35.2, which are T and B lineage lymphomas, respectively, also expressed Fgr molecules. Thus, various cells of hematopoietic origin appeared to possess a potentiality to express Fgr following activation or transformation. The present findings may help elucidate the functional significance of Fgr in immunologically committed cells in either activated or non-activated conditions.
c-fgr基因产物（Fgr）是蛋白质酪氨酸激酶src家族的成员。我们制备了一种单克隆抗体（2H2），它能识别小鼠Fgr独特的N端结构域。在本研究中，我们使用免疫组织化学分析和用2H2进行的免疫复合物激酶测定，研究了Fgr在小鼠系统中各种细胞群体和组织中的表达。在静息状态下，Fgr的表达局限于单核细胞/巨噬细胞谱系的亚群。因此，在淋巴结的副皮质区和髓质中检测到Fgr+细胞，但仅在脾脏的边缘区和胸腺的髓质中可见。在其他组织、派尔集合淋巴结、脑、心脏、肺、肝脏、胰腺、肾脏和腹腔中未检测到Fgr+巨噬细胞。然而，免疫复合物激酶测定显示，在受到刺激后，T细胞、B细胞以及腹腔巨噬细胞表达了显著水平的Fgr分子。分别为T和B谱系淋巴瘤的淋巴样起源的转化细胞系EL-4和LK35.2也表达Fgr分子。因此，各种造血起源的细胞在激活或转化后似乎具有表达Fgr的潜力。本研究结果可能有助于阐明Fgr在激活或未激活状态下的免疫相关细胞中的功能意义。
