Bringel F, Curk M C, Hubert J C
Laboratoire de Microbiologie et de Génétique URA Centre National de la Recherche Scientifique D 1481, Strasbourg, France.
Int J Syst Bacteriol. 1996 Apr;46(2):588-94. doi: 10.1099/00207713-46-2-588.
Lactobacillus plantarum, Lactobacillus pentosus, and Lactobacillus paraplantarum (M.-C. Curk, J.-C. Hubert, and F. Bringel, Int. J. Syst. Bacteriol. 46:595-598, 1996) can hardly be distinguished on the basis of their phenotypes. Unlike L. plantarum and L. paraplantarum, L. pentosus ferments glycerol and xylose but not melezitose. We identified two L. pentosus strains (CNRZ 1538 and CNRZ 1544) which ferment glycerol and melezitose but not xylose. alpha-Methyl-D-mannoside was fermented by 66% of the L. plantarum strains tested but not by L. paraplantarum strains. In this paper we describe a simple method to identify L. plantarum, L. pentosus, and L. paraplantarum. This method is based on nonradioactive Southern-type hybridization between BglI DNA digests of the lactobacilli tested and a DNA probe (L. plantarum pyrDFE genes from strain CCM 1904). A total of 68 lactobacilli were classified into five groups on the basis of the bands detected. Two groups contained L. plantarum strains; one of these groups contained 31 strains, including the type strain, and was characterized by bands at 7, 4, and 1 kb, and the other group contained strain LP 85-2 and was characterized by bands at 5 and 1.1 kb. Only one band (a band at around 7 kb) was detected in the strains belonging to the L. pentosus group, and two bands (at 4 and 1 kb) were found in the strains belonging to the L. paraplantarum group. No hybridization was detected in the last group, which contained Lactobacillus casei, Lactobacillus coryniformis, Lactobacillus paracasei, Lactobacillus brevis, Lactobacillus delbrueckii, and Lactobacillus leichmannii strains.
植物乳杆菌、戊糖乳杆菌和类植物乳杆菌（M.-C. 库尔克、J.-C. 于贝尔和F. 布林热，《国际系统细菌学杂志》46:595 - 598，1996年）很难根据其表型进行区分。与植物乳杆菌和类植物乳杆菌不同，戊糖乳杆菌能发酵甘油和木糖，但不能发酵松三糖。我们鉴定出两株戊糖乳杆菌（CNRZ 1538和CNRZ 1544），它们能发酵甘油和松三糖，但不能发酵木糖。66%的受试植物乳杆菌菌株能发酵α - 甲基 - D - 甘露糖苷，而类植物乳杆菌菌株则不能。在本文中，我们描述了一种鉴定植物乳杆菌、戊糖乳杆菌和类植物乳杆菌的简单方法。该方法基于受试乳杆菌的BglI酶切DNA与DNA探针（来自CCM 1904菌株的植物乳杆菌pyrDFE基因）之间的非放射性Southern杂交。根据检测到的条带，总共68株乳杆菌被分为五组。两组包含植物乳杆菌菌株；其中一组包含31株菌株，包括模式菌株，其特征条带位于7、4和1 kb处，另一组包含LP 85 - 2菌株，其特征条带位于5和1.1 kb处。在戊糖乳杆菌组的菌株中仅检测到一条带（约7 kb处的条带），在类植物乳杆菌组的菌株中发现了两条带（4和1 kb处）。在最后一组中未检测到杂交信号，该组包含干酪乳杆菌、棒状乳杆菌、副干酪乳杆菌、短乳杆菌、德氏乳杆菌和赖氏乳杆菌菌株。
