Love R M
Department of Restorative Dentistry, University of Otago, Dunedin, New Zealand.
J Endod. 1996 Jun;22(6):290-3. doi: 10.1016/S0099-2399(96)80261-5.
The purpose of this study was to investigate the pattern of bacterial invasion of dentinal tubules at different regions in human roots. Specimens were obtained from single-rooted teeth that had their root canals prepared in a standard manner. Roots were then sectioned longitudinally through the canals and the resulting specimens chemically treated to remove the smear layers. Specimens were immersed in a suspension of Streptococcus gordonii for 3 weeks and then prepared for histological analysis. Sections from the cervical, midroot, and apical areas were examined. The pattern of bacterial infection of the cervical and midroot areas was similar, characterized as a heavy infection with bacteria penetrating as deep as 200 microns. Invasion of the apical dentin was significantly different, with a mild infection and maximum penetration of 60 microns.
本研究的目的是调查人类牙根不同区域牙本质小管内细菌入侵的模式。标本取自单根牙，其根管按标准方式预备。然后将牙根沿根管纵向切开，对所得标本进行化学处理以去除玷污层。将标本浸入戈登链球菌悬液中3周，然后制备用于组织学分析。检查颈部、牙根中部和根尖区域的切片。颈部和牙根中部区域的细菌感染模式相似，其特征为重度感染，细菌可深入至200微米。根尖牙本质的入侵明显不同，为轻度感染，最大深度为60微米。
