Swanson M L, Collins J M, Freiman S F, Dubin N H
Department of Obstetrics and Gynecology, Union Memorial Hospital, Baltimore, Maryland 21218, USA.
J Assist Reprod Genet. 1995 Jan;12(1):48-54. doi: 10.1007/BF02214129.
This study examined sperm motion parameters as measured by computerized automated semen analysis before and after a Percoll wash and determined if differences in any parameter were correlated with fertility subsequent to intrauterine insemination (IUI).
Total motile sperm decreased following the washing procedure from 79.0 +/- 9.0 to 37.2 +/- 7.6 million sperm. Motility increased from a mean of 43.4% to 61.7% (P < 0.001). Other motility parameters also changed significantly (P < 0.001) as follows: curvilinear velocity (VCL), 43.4 to 61.7 microns/s; straight-line velocity (VSL), 21.3 to 26.7 microns/s; linearity 53.1 to 45.2%; lateral head displacement (ALH), 2.97 to 3.94 microns. Similar changes occurred following a swim-up preparation, although changes in mean motility, VCL, and ALH were significantly greater when compared to Percoll. The postwash changes were not accounted for merely by time lapse in preparation since reanalyzed untreated controls did not show the same changes in motion parameters. Prewash linearity in those specimens which resulted in pregnancies was greater than in those which did not (P = 0.28). No other significant differences in pre- or post-Percoll washed sperm motion parameters were found between pregnant vs nonfertile cycles.
Following Percoll wash all CASA-generated motility parameters were significantly altered, but there was little association between these parameters and pregnancy achieved in IUI cycles.
本研究检测了经Percoll洗涤前后通过计算机自动精液分析测得的精子运动参数，并确定任何参数的差异是否与子宫内人工授精（IUI）后的生育能力相关。
洗涤后，总活动精子数从79.0±9.0百万减少至37.2±7.6百万。活动率从平均43.4%增至61.7%（P<0.001）。其他活动参数也有显著变化（P<0.001），如下：曲线速度（VCL），从43.4微米/秒增至61.7微米/秒；直线速度（VSL），从21.3微米/秒增至26.7微米/秒；直线性从53.1%降至45.2%；头部侧摆幅度（ALH），从2.97微米增至3.94微米。上游法处理后也出现类似变化，不过与Percoll法相比，平均活动率、VCL和ALH的变化显著更大。洗涤后的变化并非仅仅由制备过程中的时间推移导致，因为重新分析未经处理的对照样本时，运动参数未显示相同变化。受孕样本的洗涤前直线性高于未受孕样本（P = 0.28）。在受孕周期与未受孕周期之间，Percoll洗涤前后的精子运动参数未发现其他显著差异。
Percoll洗涤后，所有计算机辅助精液分析生成的运动参数均有显著改变，但这些参数与IUI周期中的妊娠之间几乎没有关联。
