Inoue C, Takeshita T, Kondo H, Morimoto K
Department of Hygiene and Preventive Medicine, Osaka University School of Medicine, Japan.
Prev Med. 1996 Nov-Dec;25(6):717-24. doi: 10.1006/pmed.1996.0111.
To investigate the association between lifestyle factors and immune responses to tumors, we determined lymphokine-activated killer (LAK) cell activity and proportions of lymphocyte subsets in 54 healthy males.
LAK cell activity was determined by 51Cr release assay. The proportions of natural killer cells (CD3-16+56-, CD3-16-56+ or CD3-16+56+ cells), helper/ inducer T cells (CD4+8-), and cytotoxic/suppressor T cells (CD4-8+) in lymphocytes were also determined by flow cytometry. Subjects were classified into two groups, good or poor overall lifestyle, according to their answers on a questionnaire regarding eight health practices (cigarette smoking, alcohol consumption, physical exercise, mental stress, sleeping pattern, nutritional balance, eating breakfast, working pattern).
Subjects with good health practices regarding smoking showed significantly higher LAK cell activity. They also showed a significantly higher proportion of natural killer cells and a lower proportion of helper/inducer T cells. LAK cells activity was significantly higher in subjects with a good overall lifestyle than in those with a poor overall lifestyle after controlling for the effects of smoking. There were no significant differences in the proportions of lymphocyte subsets between the two lifestyle groups.
Lifestyle factors are associated with LAK cell activity and the proportion of lymphocyte subsets.
为了研究生活方式因素与肿瘤免疫反应之间的关联，我们测定了54名健康男性的淋巴因子激活的杀伤（LAK）细胞活性和淋巴细胞亚群比例。
通过51Cr释放试验测定LAK细胞活性。还通过流式细胞术测定淋巴细胞中自然杀伤细胞（CD3-16+56-、CD3-16-56+或CD3-16+56+细胞）、辅助/诱导性T细胞（CD4+8-）和细胞毒性/抑制性T细胞（CD4-8+）的比例。根据受试者对一份关于八项健康习惯（吸烟、饮酒、体育锻炼、精神压力、睡眠模式、营养平衡、吃早餐、工作模式）的问卷回答，将其分为两组，总体生活方式良好或不良。
在吸烟方面有良好健康习惯的受试者表现出显著更高的LAK细胞活性。他们还表现出自然杀伤细胞比例显著更高，辅助/诱导性T细胞比例更低。在控制吸烟影响后，总体生活方式良好的受试者的LAK细胞活性显著高于总体生活方式不良的受试者。两个生活方式组之间淋巴细胞亚群比例没有显著差异。
生活方式因素与LAK细胞活性和淋巴细胞亚群比例有关。
