O'Neal C L, Wolf C E, Levine B, Kunsman G, Poklis A
Department of Pathology, Medical College of Virginia, Virginia Commonwealth University, Richmond 23298-0165, USA.
Forensic Sci Int. 1996 Nov 11;83(1):31-8. doi: 10.1016/0379-0738(96)02007-5.
Three gas chromatographic procedures for the determination of ethanol in postmortem blood using alternative internal standards to n-propanol are presented: a direct injection procedure using t-butanol, and two headspace methods using t-butanol and methyl ethyl ketone. t-Butanol and methyl ethyl ketone were well resolved from ethanol, acetone, methanol and other commonly observed putrefactive volatiles using direct injection or headspace analysis. CVs for the direct injection method were below 5% for ethanol and below 10% for the other volatiles. The lower limits of detection (LOD) were 25-50 mg/L. The CVs for the headspace methods were below 5% for ethanol and below 6% for the other volatiles. The LODs were 10 mg/L using either t-butanol or methyl ethyl ketone as internal standards. The use of t-butanol or methyl ethyl ketone as alternatives to n-propanol avoids the possibility of error in the quantitation of ethanol due to the presence of n-propanol and allows for the identification of other volatiles that may aid in distinguishing antemortem ingestion from postmortem production of ethanol.
本文介绍了三种气相色谱法，用于测定死后血液中的乙醇，这些方法使用叔丁醇替代正丙醇作为内标：一种是使用叔丁醇的直接进样法，以及两种使用叔丁醇和甲乙酮的顶空法。使用直接进样或顶空分析法时，叔丁醇和甲乙酮与乙醇、丙酮、甲醇以及其他常见的腐败挥发性物质能很好地分离。直接进样法中乙醇的变异系数（CV）低于5%，其他挥发性物质的变异系数低于10%。检测限（LOD）为25 - 50 mg/L。顶空法中乙醇的变异系数低于5%，其他挥发性物质的变异系数低于6%。使用叔丁醇或甲乙酮作为内标时，检测限为10 mg/L。使用叔丁醇或甲乙酮替代正丙醇可避免因正丙醇的存在而导致乙醇定量误差的可能性，并有助于识别其他挥发性物质，这些物质可能有助于区分生前摄入乙醇与死后乙醇生成。
