Varga M, Somogyi G, Posta J, Buris L
Department of Forensic Medicine, University Medical School of Debrecen, Hungary.
Eur J Clin Chem Clin Biochem. 1993 Nov;31(11):773-6. doi: 10.1515/cclm.1993.31.11.773.
Quality assurance is an essential requirement of clinical and forensic laboratories, especially in the determination of ethanol. Opinions differ as to whether normal gas chromatographic methods should be used with modified procedures, or whether different chromatographic strategies should be adopted, the choice being based entirely on practical considerations. We have evaluated the influence on the blood ethanol determination of the frequently used glass and steel columns, as well as the use of isopropanol and tert-butanol as internal standards. All the different combinations of column type and standard provided accurate and reliable results with no significant deviation. Standardization of the analytical procedure is therefore unnecessary, but international ethanol standards should be analysed sequentially to test the method and the staff performing the analyses in the different laboratories.
质量保证是临床和法医实验室的一项基本要求，尤其是在乙醇测定方面。对于是应使用经过改进程序的常规气相色谱法，还是应采用不同的色谱策略，存在不同观点，选择完全基于实际考虑。我们评估了常用的玻璃柱和钢柱对血液乙醇测定的影响，以及使用异丙醇和叔丁醇作为内标物的情况。柱类型和标准的所有不同组合都提供了准确可靠的结果，没有明显偏差。因此，分析程序的标准化没有必要，但应依次分析国际乙醇标准，以测试方法以及不同实验室中进行分析的人员。
