Paşaoğlu H, Muhtaroğlu S, Güneş M, Utaş C
Department of Biochemistry, Erciyes University Medical Faculty, Kayseri, Turkey.
Clin Biochem. 1996 Dec;29(6):567-72. doi: 10.1016/s0009-9120(96)00097-5.
To investigate the oxidative state of glutathione and glutathione peroxidase (GSH-Px), glutathione reductase (GSSG-R), and glucose-6-phosphate dehydrogenase (G-6-PD) levels in patients with chronic renal failure (CRF) and controls.
Erythrocyte GSH levels of patients were decreased, but GSSG was not significantly different from that of controls. Also, plasma GSH levels were not different, although GSSG was increased. GSSG/GSH ratios in erythrocyte and plasma were significantly higher in CRF patients. Erythrocyte GSSG-R activity was high, but G-6-PD and GPX were low.
The findings suggest that: 1. Low GSH is related to decreased G-6-PD activities. 2. The reduction of peroxides with GPX are decreased by low GSH and low GPX activity. 3. GSSG may react with hemoglobin and causes protein aggregation in erythrocytes. These alterations cause hemolysis and could play a role in the pathogenesis of anemia in hemodialyzed patients.
研究慢性肾衰竭（CRF）患者及对照组中谷胱甘肽、谷胱甘肽过氧化物酶（GSH-Px）、谷胱甘肽还原酶（GSSG-R）以及葡萄糖-6-磷酸脱氢酶（G-6-PD）的氧化状态。
患者红细胞谷胱甘肽水平降低，但氧化型谷胱甘肽（GSSG）与对照组无显著差异。此外，血浆谷胱甘肽水平虽无差异，但GSSG升高。CRF患者红细胞和血浆中的GSSG/GSH比值显著更高。红细胞GSSG-R活性高，但G-6-PD和谷胱甘肽过氧化物酶（GPX）活性低。
研究结果表明：1. 低谷胱甘肽与G-6-PD活性降低有关。2. 低谷胱甘肽和低GPX活性导致GPX对过氧化物的还原作用降低。3. GSSG可能与血红蛋白反应并导致红细胞中的蛋白质聚集。这些改变会引起溶血，并可能在血液透析患者贫血的发病机制中起作用。
