Canestrari F, Galli F, Giorgini A, Albertini M C, Galiotta P, Pascucci M, Bossù M
G. Fornaini Institut of Biochemistry, University of Urbino, Italy.
Acta Haematol. 1994;91(4):187-93. doi: 10.1159/000204332.
Reduced and oxidized glutathione and pyridine coenzymes, glutathione-related enzymes and Cu,Zn-superoxide dismutase (Cu,Zn-SOD) were investigated in the RBC of patients with chronic renal failure (CRF) and in age- and sex-matched controls. The effects of hemodialysis (HD) were also studied. A defective RBC redox state was shown in the CRF group based on a decreased GSH/GSSG ratio and NADPH levels. Increased activities of glutathione transferase (GSH-S-T) and Cu,Zn-SOD were observed before HD. Dialysis apparently restores the levels of antioxidant enzymes and at the same time strongly affects the redox state. Thus we can speculate that HD can generate severe redox impairment inducing damage in RBC and plasma antioxidant enzymes. Increased erythrocyte GSSG and GSM-S-T levels coupled with a reduced hexose monophosphate shunt (HMPS) function may be useful indexes of oxidative stress in uremic anemia.
对慢性肾衰竭（CRF）患者以及年龄和性别匹配的对照组的红细胞中的还原型和氧化型谷胱甘肽、吡啶辅酶、谷胱甘肽相关酶和铜锌超氧化物歧化酶（Cu,Zn-SOD）进行了研究。还研究了血液透析（HD）的影响。基于谷胱甘肽（GSH）/氧化型谷胱甘肽（GSSG）比值和烟酰胺腺嘌呤二核苷酸磷酸（NADPH）水平降低，CRF组显示出红细胞氧化还原状态存在缺陷。在血液透析前观察到谷胱甘肽转移酶（GSH-S-T）和铜锌超氧化物歧化酶的活性增加。透析显然能恢复抗氧化酶的水平，同时强烈影响氧化还原状态。因此我们可以推测，血液透析会产生严重的氧化还原损伤，导致红细胞和血浆抗氧化酶受损。红细胞GSSG和谷胱甘肽转移酶水平升高，同时己糖磷酸旁路（HMPS）功能降低，可能是尿毒症贫血氧化应激的有用指标。
