Paschalis E P, DiCarlo E, Betts F, Sherman P, Mendelsohn R, Boskey A L
The Hospital for Special Surgery, New York, New York, USA.
Calcif Tissue Int. 1996 Dec;59(6):480-7. doi: 10.1007/BF00369214.
Fourier Transform Infrared Microspectroscopy (FTIRM) has been used to study the changes in mineral and matrix content and composition in replicate biopsies of nonosteoporotic human osteonal bone. Spectral maps in four orthogonal directions (in 10 microm steps) from the centers towards the peripheries of individual osteons were obtained from iliac crest biopsies of two necropsy cases. Mineral to matrix ratios, calculated from the ratio of integrated areas of the phosphate nu1,nu3 band at 900-1200 cm-1 to the amide I band at 1585-1725 cm-1, increased from the center to the periphery of the osteon. The total carbonate (based on the nu2 band at approximately 850-900 cm-1) to phosphate nu1,nu3 ratio decreased as the mineral to matrix ratio increased. Analysis of the nu2 CO32- band with a combination of second-derivative spectroscopy and curve fitting revealed a decrease in "labile" carbonate, a slight decrease in Type A and a slight increase in Type B carbonate from the center to the periphery of the osteon. Similar analysis of the components of the nu1,nu3 phosphate band with a combination of second-derivative spectroscopy and curve fitting revealed the presence of 11 major underlying moieties. These components were assigned by comparison with published frequencies for apatite and acid-phosphate containing calcium phosphates. The most consistent variations were alterations in the relative percent areas of bands at approximately 1020 and approximately 1030 cm-1, which had previously been assigned to nonstoichiometric and stoichiometric apatites, respectively. This ratio was used as an index of variation in crystal perfection throughout the osteon. This ratio decreased as the mineral to matrix ratio increased. The reproducibility of these parameters at multiple sites in multiple biopsies suggests their applicability for the analysis of mineral changes in disease.
傅里叶变换红外显微光谱法（FTIRM）已被用于研究非骨质疏松性人类骨单位骨重复活检中矿物质和基质含量及成分的变化。从两例尸检病例的髂嵴活检中获取了单个骨单位从中心到周边四个正交方向（步长为10微米）的光谱图。通过计算900 - 1200 cm-1处磷酸盐ν1、ν3带积分面积与1585 - 1725 cm-1处酰胺I带积分面积的比值得到矿物质与基质的比率，该比率从骨单位中心向周边增加。总碳酸盐（基于约850 - 900 cm-1处的ν2带）与磷酸盐ν1、ν3的比率随着矿物质与基质比率的增加而降低。结合二阶导数光谱和曲线拟合对ν2 CO32-带进行分析，结果显示从骨单位中心到周边，“不稳定”碳酸盐减少，A型碳酸盐略有减少，B型碳酸盐略有增加。结合二阶导数光谱和曲线拟合对ν1、ν3磷酸盐带的成分进行类似分析，结果显示存在11个主要潜在部分。通过与已发表的磷灰石和含酸磷酸盐的磷酸钙频率进行比较对这些成分进行了归属。最一致的变化是约1020 cm-1和约1030 cm-1处谱带相对面积百分比的改变，此前分别将其归属为非化学计量磷灰石和化学计量磷灰石。该比率被用作整个骨单位晶体完善程度变化的指标。该比率随着矿物质与基质比率的增加而降低。这些参数在多个活检的多个部位的可重复性表明它们适用于疾病中矿物质变化的分析。
