细胞毒性、溶血性豚鼠气单胞菌临床分离株的特性鉴定及其通过检测独特溶血素基因的存在进行识别。
Characterization of cytotoxic, hemolytic Aeromonas caviae clinical isolates and their identification by determining presence of a unique hemolysin gene.
作者信息
Wang G, Tyler K D, Munro C K, Johnson W M
机构信息
Bureau of Microbiology, Laboratory Centre for Disease Control, Health Canada, Ottawa, Ontario, Canada.
出版信息
J Clin Microbiol. 1996 Dec;34(12):3203-5. doi: 10.1128/jcm.34.12.3203-3205.1996.
Abstract
Aeromonas caviae has recently been recognized as an important enteropathogen and its hemolysin is purported to be one of the virulence factors, In this study, a total of 80 clinical isolates of Aeromonas spp. were investigated by PCR with synthetic oligonucleotides targeting a cloned hemolysin-encoding sequence from an A. caviae isolate of clinical origin. Of the 35 clinical A. caviae isolates tested, only 6 contained the target sequence.
摘要
豚鼠气单胞菌最近被认为是一种重要的肠道病原体，其溶血素被认为是毒力因子之一。在本研究中，使用针对来自临床来源的豚鼠气单胞菌分离株的克隆溶血素编码序列的合成寡核苷酸，通过PCR对总共80株气单胞菌属临床分离株进行了研究。在测试的35株临床豚鼠气单胞菌分离株中，只有6株含有目标序列。
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本文引用的文献
1
Haemagglutinating activity, serum sensitivity and enterotoxigenicity of Aeromonas spp.气单胞菌属的血凝活性、血清敏感性及产肠毒素性
J Med Microbiol. 1993 Jan;38(1):49-53. doi: 10.1099/00222615-38-1-49.
2
Characterisation of haemolytic activity from Aeromonas caviae.豚鼠气单胞菌溶血活性的表征
Epidemiol Infect. 1994 Apr;112(2):291-8. doi: 10.1017/s0950268800057708.
3
Detection of enterotoxins of Aeromonas hydrophila by a suckling-mouse test.通过乳鼠试验检测嗜水气单胞菌肠毒素
J Med Microbiol. 1981 Nov;14(4):401-8. doi: 10.1099/00222615-14-4-401.
4
Aeromonas hydrophila: ecology and toxigenicity of isolates from an estuary.嗜水气单胞菌：来自河口分离株的生态学与产毒性
J Appl Bacteriol. 1981 Apr;50(2):359-77. doi: 10.1111/j.1365-2672.1981.tb00900.x.
5
Aeromonas septicemia in infants and children.婴幼儿气单胞菌败血症
Pediatr Infect Dis. 1984 Mar-Apr;3(2):122-5. doi: 10.1097/00006454-198403000-00008.
6
Bacteremia caused by Aeromonas species in hospitalized cancer patients.住院癌症患者中由气单胞菌属引起的菌血症。
Rev Infect Dis. 1985 May-Jun;7(3):314-20. doi: 10.1093/clinids/7.3.314.
7
Partial characterisation of a soluble haemagglutinin from human diarrhoeal isolates of Aeromonas.来自气单胞菌属人类腹泻分离株的一种可溶性血凝素的部分特性分析
J Med Microbiol. 1986 Jun;21(4):319-24. doi: 10.1099/00222615-21-4-319.
8
Nucleotide sequence of the gene for the hole-forming toxin aerolysin of Aeromonas hydrophila.嗜水气单胞菌成孔毒素气溶素基因的核苷酸序列。
J Bacteriol. 1987 Jun;169(6):2869-71. doi: 10.1128/jb.169.6.2869-2871.1987.
9
Mesophilic aeromonads in human disease: current taxonomy, laboratory identification, and infectious disease spectrum.人类疾病中的嗜温气单胞菌：当前的分类学、实验室鉴定及传染病谱
Rev Infect Dis. 1988 Sep-Oct;10(5):980-97. doi: 10.1093/clinids/10.5.980.
10
Cloning of the Vibrio cholerae recA gene and construction of a Vibrio cholerae recA mutant.霍乱弧菌recA基因的克隆及霍乱弧菌recA突变体的构建。
J Bacteriol. 1986 Mar;165(3):715-22. doi: 10.1128/jb.165.3.715-722.1986.
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