Pinteaux E, Copin J C, Ledig M, Tholey G
Laboratoire de Neurobiologie Ontogénique, ERS 110 du CNRS, Strasbourg, France.
Dev Neurosci. 1996;18(5-6):397-404. doi: 10.1159/000111433.
Cytosolic and mitochondrial alterations induced by exposure of rat astroglial primary cultures to reactive oxygen species (ROS) generated by a xanthine/xanthine oxidase (X/XO) mixture or by lipopolysaccharide (LPS) have been investigated biochemically and immunochemically. In the presence of ROS generated by X/XO, a significant decrease in Cu,Zn superoxide dismutase (Cu,Zn-SOD) and in glutamine synthetase (GS) activity was observed whereas mitochondrial Mn-SOD activity and enzyme protein levels were significantly enhanced. Similar effects on GS, Cu,Zn- and Mn-SOD activities were observed by glucose/glucose oxidase treatment of the cells. Addition of LPS to the cell growth medium also specifically induces Mn-SOD synthesis but was without effect on Cu,Zn-SOD. It is suggested that in all these tested situations, hydrogen peroxide could represent a specific inducer of the observed phenomenon and it may therefore be considered as an intracellular messenger involved in the regulation of some aspects of astroglial oxidative metabolism, particularly the defence against ROS.
通过生物化学和免疫化学方法，研究了原代大鼠星形胶质细胞培养物暴露于黄嘌呤/黄嘌呤氧化酶（X/XO）混合物或脂多糖（LPS）产生的活性氧（ROS）所诱导的胞质和线粒体变化。在X/XO产生的ROS存在下，观察到铜锌超氧化物歧化酶（Cu,Zn-SOD）和谷氨酰胺合成酶（GS）活性显著降低，而线粒体锰超氧化物歧化酶（Mn-SOD）活性和酶蛋白水平显著增强。通过葡萄糖/葡萄糖氧化酶处理细胞，观察到对GS、Cu,Zn-SOD和Mn-SOD活性有类似影响。向细胞生长培养基中添加LPS也特异性诱导Mn-SOD合成，但对Cu,Zn-SOD无影响。提示在所有这些测试情况下，过氧化氢可能是观察到的现象的特异性诱导物，因此可被视为参与星形胶质细胞氧化代谢某些方面调节的细胞内信使，特别是对ROS的防御。
