Department of Pharmacology, School of Pharmacy, Universidad Complutense de Madrid, Madrid, Spain.
Plant Foods Hum Nutr. 2009 Dec;64(4):238-43. doi: 10.1007/s11130-009-0137-9.
Phenolic composition of wine depends not only on the grape variety from which it is made, but on some external factors such as winemaking technology. Red wine possesses the most antioxidant effect because of its high polyphenolic content. The aim of this work is to study for the first time, the neuroprotective activity of four monovarietal Spanish red wines (Merlot (ME), Tempranillo (T), Garnacha (G) and Cabernet-Sauvignon (CS)) through its antioxidant ability, and to relate this neuroprotection to its polyphenolic composition, if possible. The wine effect on neuroprotection was studied through its effect as free radical scavenger against FeSO4, H2O2 and FeSO4 + H2O2. Effect on cell survival was determined by 3(4,5-dimethyltiazol-2-il)-2,5-diphenyltetrazolium reduction assay (MTT) and lactate dehydrogenase (LDH) release assay on astrocytes cultures. Results showed that most of the studied wine varieties induced neuroprotection through their antioxidant ability in astrocytes, Merlot being the most active; this variety is especially rich in phenolic compounds, mainly catechins and oligomeric proanthocyanidins. Our results show that red wine exerts a protection against oxidative stress generated by different toxic agents and that the observed neuroprotective activity is related to their polyphenolic content.
葡萄酒的酚类成分不仅取决于酿造它的葡萄品种，还取决于一些外部因素，如酿酒技术。由于其多酚含量高，红葡萄酒具有最强的抗氧化作用。本工作的目的是首次研究四种西班牙单一品种红葡萄酒（梅洛（ME）、丹魄（T）、歌海娜（G）和赤霞珠（CS））的神经保护活性，通过其抗氧化能力，并尽可能将这种神经保护作用与其多酚成分联系起来。通过其对 FeSO4、H2O2 和 FeSO4+H2O2 的自由基清除能力来研究葡萄酒对神经保护的作用。通过 3（4,5-二甲基噻唑-2-基）-2,5-二苯基四唑（MTT）减少法和乳酸脱氢酶（LDH）释放法在星形胶质细胞培养物上测定细胞存活率。结果表明，研究的大多数葡萄酒品种通过其在星形胶质细胞中的抗氧化能力诱导神经保护作用，梅洛最为活跃；这种品种特别富含酚类化合物，主要是儿茶素和低聚原花青素。我们的结果表明，红葡萄酒对不同毒性剂产生的氧化应激具有保护作用，并且观察到的神经保护活性与其多酚含量有关。
