Koseki T, Benno Y, Zhang-Koseki Y J, Umeda M, Ishikawa I
Dentistry of Periodontology, Faculty of Dentistry, Tokyo Medical and Dental University, Japan.
Oral Microbiol Immunol. 1996 Jun;11(3):203-8. doi: 10.1111/j.1399-302x.1996.tb00359.x.
Selected media were compared for primary isolation and detection of oral treponemes from clinical samples. Forty-eight subgingival plaque samples from 45 patients suffering from periodontitis were anaerobically cultivated for 2 weeks at 37 degrees C. Of the 9 media studied, Medium 10 (M10), which was supplemented with 10% rabbit serum and incubated using the plate-in-bottle method, supported the highest colony-forming units of the anaerobes. The treponemal colonies were detected at least on one medium from 83% of the subgingival plaque samples. The new oral spirochete medium in an anaerobic chamber supported the highest detection frequency of the oral treponemes (64% of samples); however, M10 in the plate-in-bottle was found to produce the highest colony-forming unit recovery of the oral treponemes (median 3.6% of the total colony-forming units). This study suggests that M10 in the plate-in-bottle and new oral spirochete medium in the anaerobic chamber are essential in cultivating oral treponemes.
对用于从临床样本中进行口腔密螺旋体初代分离和检测的选定培养基进行了比较。对45例牙周炎患者的48份龈下菌斑样本在37℃下厌氧培养2周。在所研究的9种培养基中，添加10%兔血清并采用瓶内平板法孵育的培养基10（M10）支持厌氧菌形成的菌落形成单位数量最多。在83%的龈下菌斑样本中，至少在一种培养基上检测到了密螺旋体菌落。厌氧箱中的新型口腔螺旋体培养基支持口腔密螺旋体的最高检测频率（样本的64%）；然而，发现瓶内平板法中的M10产生的口腔密螺旋体菌落形成单位回收率最高（占总菌落形成单位的中位数为3.6%）。本研究表明，瓶内平板法中的M10和厌氧箱中的新型口腔螺旋体培养基对于培养口腔密螺旋体至关重要。
