Koseki T, Ishikawa I, Boutsi E, He T, Benno Y
Department of Periodontology, Faculty of Dentistry, Tokyo Medical and Dental University, Japan.
Oral Microbiol Immunol. 1996 Jun;11(3):166-71. doi: 10.1111/j.1399-302x.1996.tb00353.x.
In order to cultivate fermentative treponemes isolated on Medium 10 with the plate-in-bottle method from subgingival plaque, we developed the enriched Medium 10. Enriched Medium 10 broth consisted mainly of Trypticase peptone (2 g/L), yeast extract (10 g/l), glucose (5 g/l) and volatile fatty acids mixture (3.1 ml/l). The growth curves in enriched Medium 10 broth showed that clinical isolates of Treponema socranskii reached the early stationary phase on the 3rd-5th day, and the doubling times averaged 22.2h. By using enriched Medium 10, the nutritional analysis of oral treponemes was examined for each compound of the volatile fatty acid and various sera. Clinical isolates were found to require iso-butyric acid, 2-methylbutyric acids and/or iso-caproic acid independently for their growth. However, the growth of these fermentative treponemes was inhibited by addition of various sera to this medium.
为了用瓶内平板法培养从龈下菌斑中分离于10号培养基上的发酵密螺旋体，我们研发了改良10号培养基。改良10号肉汤培养基主要由胰蛋白酶胨（2 g/L）、酵母提取物（10 g/l）、葡萄糖（5 g/l）和挥发性脂肪酸混合物（3.1 ml/l）组成。改良10号肉汤培养基中的生长曲线表明，索氏密螺旋体临床分离株在第3 - 5天进入早期稳定期，平均倍增时间为22.2小时。通过使用改良10号培养基，对挥发性脂肪酸的每种化合物和各种血清进行了口腔密螺旋体的营养分析。发现临床分离株生长分别需要异丁酸、2 - 甲基丁酸和/或异己酸。然而，向该培养基中添加各种血清会抑制这些发酵密螺旋体的生长。
