van Leeuwen P, Veldman A, Boisen S, Deuring K, Van Kempen G J, Derksen G B, Verstegen M W, Schaafsma G
TNO Nutrition and Food Research, Department of Animal Nutrition and Meat Technology, Wageningen, The Netherlands.
Br J Nutr. 1996 Oct;76(4):551-62. doi: 10.1079/bjn19960062.
Two experiments were conducted to determine apparent ileal DM and crude-protein (CP) digestibilities in rations fed to pigs. An evaluation was made of Cr2O3 and HCl-insoluble ash as digestive markers. In addition, the effects of body weight (BW) on apparent ileal DM and CP (N x 6.25) digestibilities were studied. In Expt 1, thirteen barrows averaging 35 kg BW were fitted with post-valve T-caecum (PVTC) cannulas to determine the apparent ileal DM and CP digestibilities of a wheat gluten-bran ration (B2) and a soyabean-meal ration (E1). Immediately after morning feeding ileal digesta samples were collected on an hourly basis for a total of 12 h. Subsequently. N and marker contents were determined in the samples. The postprandial patterns of N and Cr passage were more similar than those of N and HCl-insoluble ash. Therefore Cr2O3 is more suitable as a marker than HCl-insoluble ash. The apparent ileal CP digestibility coefficient of ration B2 derived using Cr2O3 as a marker was significantly (P < 0.05) higher by 0.018 compared with the value obtained using HCl-insoluble ash. The corresponding values for ration E2 obtained using Cr2O3 and HCl-insoluble ash were both 0.825. In Expt 2, apparent ileal DM and CP digestibilities were determined in eighteen rations using twelve barrows also fitted with PVTC cannulas (BW from 40 to 100 kg). The protein sources for these rations were from different groups of feedstuffs. In four and three of the rations apparent ileal DM and CP digestibilities respectively were significantly different (P < 0.05) when assessed using the two markers. The digestibility coefficients were not systematically higher or lower for either marker. Absolute differences were < 0.049 on average. Significant effects of live weight on apparent ileal CP digestibilities were found.
进行了两项试验以测定猪日粮中回肠表观干物质(DM)和粗蛋白(CP)消化率。对三氧化二铬(Cr2O3)和盐酸不溶性灰分作为消化标记物进行了评估。此外,研究了体重(BW)对回肠表观干物质和粗蛋白(N×6.25)消化率的影响。在试验1中,13头平均体重35 kg的公猪安装了回肠末端-盲肠(PVTC)套管,以测定小麦面筋-麸皮日粮(B2)和豆粕日粮(E1)的回肠表观干物质和粗蛋白消化率。早晨喂食后立即每小时采集一次回肠食糜样本,共采集12小时。随后,测定样本中的氮和标记物含量。氮和铬的餐后通过模式比氮和盐酸不溶性灰分的餐后通过模式更相似。因此,与盐酸不溶性灰分相比,三氧化二铬更适合作为标记物。使用三氧化二铬作为标记物得出的日粮B2的回肠表观粗蛋白消化率系数比使用盐酸不溶性灰分获得的值显著(P<0.05)高0.018。使用三氧化二铬和盐酸不溶性灰分获得的日粮E2的相应值均为0.825。在试验2中,使用12头同样安装了PVTC套管(体重40至100 kg)的公猪,测定了18种日粮的回肠表观干物质和粗蛋白消化率。这些日粮的蛋白质来源来自不同组别的饲料原料。在其中4种和3种日粮中,使用两种标记物评估时,回肠表观干物质和粗蛋白消化率分别存在显著差异(P<0.05)。两种标记物的消化率系数没有系统性地更高或更低。平均绝对差异<0.049。发现活重对回肠表观粗蛋白消化率有显著影响。