Lesage G, Glaser S S, Gubba S, Robertson W E, Phinizy J L, Lasater J, Rodgers R E, Alpini G
Department of Internal Medicine, Scott & White Hospital, Temple, Texas, USA.
Gastroenterology. 1996 Dec;111(6):1633-44. doi: 10.1016/s0016-5085(96)70027-6.
BACKGROUND & AIMS: After partial hepatectomy, liver regeneration occurs with the return of hepatocyte mass to normal, Limited data exist regarding the renewal of the biliary tree after partial hepatectomy. This study tested the hypothesis that, after partial hepatectomy, the biliary tree regenerates by proliferation of the remaining cholangiocytes, leading to an increase in secretin-induced ductal bile secretion.
After 70% partial hepatectomy, cholangiocyte proliferation was assessed in situ by morphometric analysis and In vitro by measurement of 3H-thymidine incorporation. Ductal secretion was estimated by measurement of secretin receptor gene expression and adenosine 3',5'-cyclic monophosphate (cAMP) levels in vitro and by the effect of secretin on ductal bile secretion in vivo.
DNA synthesis was undetectable in control cholangiocytes, increased and peaked at day 3 after partial hepatectomy, and returned to normal by day 28. Morphometric analysis showed regrowth of the biliary tree beginning at day 1 with restoration by day 10. The expression of secretin receptor gene and secretin-induced cAMP levels and secretin-induced bicarbonate-rich choleresis increased during the period of bile duct renewal.
After partial hepatectomy, the increase in secretin-induced ductal bile secretion observed during bile duct renewal results from proliferation of remaining cholangiocytes.
部分肝切除术后，肝细胞数量恢复正常，肝脏发生再生。关于部分肝切除术后胆管树更新的数据有限。本研究检验了以下假设：部分肝切除术后，胆管树通过剩余胆管细胞的增殖而再生，导致促胰液素诱导的胆管胆汁分泌增加。
在70%部分肝切除术后，通过形态计量分析在原位评估胆管细胞增殖，并通过测量³H-胸腺嘧啶核苷掺入在体外评估。通过测量促胰液素受体基因表达和体外3',5'-环磷酸腺苷（cAMP）水平，以及通过促胰液素对体内胆管胆汁分泌的影响来估计胆管分泌。
在对照胆管细胞中未检测到DNA合成，部分肝切除术后第3天DNA合成增加并达到峰值，到第28天恢复正常。形态计量分析显示胆管树在第1天开始再生，并在第10天恢复。在胆管更新期间，促胰液素受体基因的表达、促胰液素诱导的cAMP水平以及促胰液素诱导的富含碳酸氢盐的胆汁分泌增加。
部分肝切除术后，在胆管更新期间观察到的促胰液素诱导的胆管胆汁分泌增加是由于剩余胆管细胞的增殖。
