Dockery P, Pritchard K, Warren M A, Li T C, Cooke I D
Department of Anatomy, University College, Cork, Ireland.
Hum Reprod. 1996 Oct;11(10):2251-6. doi: 10.1093/oxfordjournals.humrep.a019085.
In the light and electron microscopical study reported here, we documented the structure of the nucleus, nucleolus and nuclear channel system (NCS) in the uterine glandular epithelium in both fertile and infertile women during the early luteal phase. Nuclear volume was found to be larger in the infertile group at day 5 after the luteinizing hormone surge (LH+5) compared to day-matched fertile subjects. A two-way analysis of variance performed on nucleolar volume data from fertile and infertile women biopsied on days LH+5, +5, and +6 revealed a significant effect of condition but no effect of day or interaction. Nucleolar volume decreased from day LH+2 to day LH+6 in the fertile group, the sharpest decrease occurring between day LH+3 and day LH+4. The largest mean volume of the NCS was found at day LH+5 in the fertile group and day LH+6 in the infertile group. The results suggest a delay in the development of this organelle in the infertile women. The present study has documented alterations in the nuclei of uterine glandular cells from infertile patients. In these infertile women, there is also a delayed elaboration of the secretory apparatus and this delay correlates well with the delayed/reduced expression of a luteal-specific glyco-protein.
在本文报道的光镜和电镜研究中，我们记录了黄体期早期有生育能力和无生育能力女性子宫腺上皮细胞核、核仁及核通道系统（NCS）的结构。与年龄匹配的有生育能力的受试者相比，在促黄体生成素激增后第5天（LH + 5），无生育能力组的细胞核体积更大。对在LH + 5、+ 5和+ 6天进行活检的有生育能力和无生育能力女性的核仁体积数据进行双向方差分析，结果显示状态有显著影响，但天数或交互作用无影响。在有生育能力组中，核仁体积从LH + 2天到LH + 6天逐渐减小，在LH + 3天到LH + 4天之间下降最为明显。有生育能力组在LH + 5天、无生育能力组在LH + 6天发现核通道系统的平均体积最大。结果表明无生育能力女性该细胞器的发育存在延迟。本研究记录了无生育能力患者子宫腺细胞的细胞核变化。在这些无生育能力女性中，分泌装置的发育也延迟，且这种延迟与黄体特异性糖蛋白表达的延迟/降低密切相关。
