Sir William Dunn School of Pathology, University of Oxford, Oxford OX1 3RE, UK.
Weill Cornell Medical College, Cornell University, New York, NY 10065, USA.
Int J Mol Sci. 2019 Nov 20;20(23):5839. doi: 10.3390/ijms20235839.
The nuclei of cells may exhibit invaginations of the nuclear envelope under a variety of conditions. These invaginations form a branched network termed the nucleoplasmic reticulum (NR), which may be found in cells in pathological and physiological conditions. While an extensive NR is a hallmark of cellular senescence and shows associations with some cancers, very little is known about the formation of NR in physiological conditions, despite the presence of extensive nuclear invaginations in some cell types such as endometrial cells. Here we show that in these cells the NR is formed in response to reproductive hormones. We demonstrate that oestrogen and progesterone are sufficient to induce NR formation and that this process is reversible without cell division upon removal of the hormonal stimulus. Nascent lamins and phospholipids are incorporated into the invaginations suggesting that there is a dedicated machinery for its formation. The induction of NR in endometrial cells offers a new model to study NR formation and function in physiological conditions.
细胞的核可能在各种条件下表现出核膜的内陷。这些内陷形成了一种分支的网络，称为核质网状结构（NR），可以在病理和生理条件下的细胞中找到。虽然广泛的 NR 是细胞衰老的标志，并与一些癌症有关，但对于生理条件下 NR 的形成知之甚少，尽管某些细胞类型如子宫内膜细胞存在广泛的核内陷。在这里，我们表明在这些细胞中，NR 是响应生殖激素形成的。我们证明雌激素和孕激素足以诱导 NR 的形成，并且在去除激素刺激后，无需细胞分裂，这个过程是可逆的。新生的核纤层蛋白和磷脂被纳入内陷中，表明存在专门的形成机制。在子宫内膜细胞中诱导 NR 的形成提供了一个新的模型来研究生理条件下 NR 的形成和功能。
