Divakaran P, Venkataraman A
J Nutr. 1977 Sep;107(9):1621-31. doi: 10.1093/jn/107.9.1621.
Hydrogenated coconut oil or safflower seed oil were fed at 20% levels to weanling male albino rats for 2 months. The fatty acid patterns of the liver homogenates, mitochondria and the microsomes were determined by gas chromatography as were also the fatty acid patterns of the liver cholesterol esters and the phospholipids. The mitochondrial phospholipids were fractionated by thin layer chromatography and the fatty acid moieties of the individual phospholipids were screened on a gas chromatograph. The oxidative phosphorylation in the liver mitochondria was determined using glutamate, malate and succinate as substrates. The liver fatty acid pattern, especially that of the subcellular particles, seemed to be dependent upon the dietary fat. The fatty acid composition of the mitochondrial phospholipids varied with the dietary fat. Oxidative phosphorylation for glutamate and malate was higher in the group fed safflower oil compared to that in the group fed saturated fat; in the case of succinate, no such difference was noticed. These results suggest that the changes in the phosphorylation capacity are due to the changes in the mitochondrial phospholipids which reflect the composition of the dietary fat.
将氢化椰子油或红花籽油以20%的比例喂给断乳雄性白化大鼠，持续2个月。通过气相色谱法测定肝脏匀浆、线粒体和微粒体的脂肪酸模式，以及肝脏胆固醇酯和磷脂的脂肪酸模式。通过薄层色谱法分离线粒体磷脂，并在气相色谱仪上筛选各个磷脂的脂肪酸部分。以谷氨酸、苹果酸和琥珀酸为底物，测定肝脏线粒体中的氧化磷酸化。肝脏脂肪酸模式，尤其是亚细胞颗粒的脂肪酸模式，似乎取决于膳食脂肪。线粒体磷脂的脂肪酸组成随膳食脂肪而变化。与喂食饱和脂肪的组相比，喂食红花油的组中谷氨酸和苹果酸的氧化磷酸化更高；对于琥珀酸，未观察到这种差异。这些结果表明，磷酸化能力的变化是由于线粒体磷脂的变化，而线粒体磷脂的变化反映了膳食脂肪的组成。
