幽门螺杆菌酶表达最佳液体培养基的测定
Determination of optimal liquid medium for enzyme expression by Helicobacter pylori.
作者信息
Lin Y L, Lee N, Chan E C
机构信息
Medical Biotechnology Laboratory, School of Medical Technology, Chang Gung College of Medicine and Technology, Kweishan, Taoyuan, Republic of China.
出版信息
J Clin Pathol. 1996 Oct;49(10):818-20. doi: 10.1136/jcp.49.10.818.
AIMS
To determine the best medium for the growth and enzyme expression of Helicobacter pylori.
METHODS
Twelve H pylori isolates from histologically confirmed infected patients were cultured on Brucella broth (BB), Brucella broth minus bisulphite (BLBB), and brain-heart infusion broth (BHIB), all supplemented with 5% (v/v) fetal calf serum. Growth rates and enzyme expressions of all H pylori isolates cultivated in these media were evaluated.
RESULTS
Although both BLBB and BHIB supported good growth of H pylori under microaerophilic conditions, the total protein content of H pylori detected was much higher with BHIB cultivation. Measurement of the specific activities of urease, phospholipase C, and sphingomyelinase for 12 H pylori isolates cultivated in these media, showed that BHIB supported the highest expression of these enzymes. Although BLBB supported better growth of H pylori than BB, it did not increase enzyme expression.
CONCLUSIONS
Cultivation of H pylori in BHIB is recommended for studies on the physiology, metabolism, and enzyme expression of the organism.
目的
确定幽门螺杆菌生长和酶表达的最佳培养基。
方法
从组织学确诊感染患者中分离出的12株幽门螺杆菌，在添加5%（v/v）胎牛血清的布鲁氏菌肉汤（BB）、不含亚硫酸盐的布鲁氏菌肉汤（BLBB）和脑心浸液肉汤（BHIB）中培养。评估在这些培养基中培养的所有幽门螺杆菌分离株的生长速率和酶表达。
结果
虽然BLBB和BHIB在微需氧条件下均支持幽门螺杆菌良好生长，但用BHIB培养时检测到的幽门螺杆菌总蛋白含量要高得多。对在这些培养基中培养的12株幽门螺杆菌分离株的脲酶、磷脂酶C和鞘磷脂酶的比活性进行测定，结果表明BHIB支持这些酶的最高表达。虽然BLBB比BB更有利于幽门螺杆菌生长，但它并未增加酶表达。
结论
推荐在BHIB中培养幽门螺杆菌用于该生物体的生理学、代谢和酶表达研究。
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