Piérard G E, Piérard-Franchimont C, Nikkels A, Nikkels-Tassoudji N, Arrese J E, Bourguignon J P
Department of Dermatopathology and Pediatrics, University of Liège, Belgium.
J Pathol. 1996 Sep;180(1):74-9. doi: 10.1002/(SICI)1096-9896(199609)180:1<74::AID-PATH595>3.0.CO;2-A.
The influence of growth hormone and insulin-like growth factor I on human melanocytes is being increasingly recognized. Clinical evidence has shown that when recombinant human growth hormone (hGH) is administered to children of short stature, the growth of melanocytic naevi is boosted. This study was conducted on 56 hGH-triggered naevi and nine similar lesions excised before or after hGH therapy for hypopituitarism and Turner's syndrome. A series of 40 naevi excised from age-matched healthy children served as controls. Atypicality of naevocytes was investigated using image analysis, AgNOR counts, immunohistochemistry (HMB-45, NKI-C3, Ki-67, anti-bcl-2-oncoprotein), and DNA flow cytometry. The data associate hGH treatment with anisokaryosis and increased AgNOR and Ki-67 counts in naevocytes. The same cells also show abnormal patterns of HMB-45 immunolabelling. These indications of naevocyte activation were not suggestive of malignant transformation. hGH-triggered melanocytomas should be added to the list of atypical melanocytic naevi. The long-term evolution of these lesions remains unknown and the potential risk of malignant transformation awaits careful evaluation.
生长激素和胰岛素样生长因子I对人类黑素细胞的影响日益受到认可。临床证据表明，给身材矮小的儿童注射重组人生长激素（hGH）时，黑素细胞痣的生长会加快。本研究对56例由hGH引发的痣以及9例在垂体功能减退和特纳综合征的hGH治疗前后切除的类似病变进行了研究。从年龄匹配的健康儿童身上切除的40例痣作为对照。使用图像分析、核仁组成区嗜银蛋白计数、免疫组织化学（HMB - 45、NKI - C3、Ki - 67、抗bcl - 2癌蛋白）和DNA流式细胞术研究痣细胞的非典型性。数据表明hGH治疗与痣细胞的核大小不等以及核仁组成区嗜银蛋白和Ki - 67计数增加有关。同样的细胞还显示出HMB - 45免疫标记的异常模式。这些痣细胞激活的迹象并不提示恶性转化。由hGH引发的黑素细胞瘤应被列入非典型黑素细胞痣名单。这些病变的长期演变尚不清楚，恶性转化的潜在风险有待仔细评估。
