Zychlinsky A, Thirumalai K, Arondel J, Cantey J R, Aliprantis A O, Sansonetti P J
The Skirball Institute, New York University School of Medicine, New York, New York 10016, USA.
Infect Immun. 1996 Dec;64(12):5357-65. doi: 10.1128/iai.64.12.5357-5365.1996.
Shigella flexneri, an etiological agent of bacillary dysentery, causes apoptosis in vitro. Here we show that it also induces apoptosis in vivo. We were able to quantify the number of apoptotic cells in rabbit Peyer's patches infected with S. flexneri by detecting cells with fragmented DNA. Infection with virulent S. flexneri results in massive numbers of apoptotic cells within the lymphoid follicles. In contrast, neither an avirulent strain nor an avirulent strain capable of colonizing Peyer's patches increases the background level of apoptotic cells. Macrophages, T cells, and B cells are shown to undergo apoptosis in vivo. These results indicate that apoptosis may play a crucial role in the pathogenesis of shigellosis.
福氏志贺菌是细菌性痢疾的病原体，可在体外诱导细胞凋亡。在此我们表明它也能在体内诱导细胞凋亡。通过检测DNA片段化的细胞，我们能够对感染福氏志贺菌的兔派伊尔结中的凋亡细胞数量进行量化。感染强毒力的福氏志贺菌会导致淋巴滤泡内出现大量凋亡细胞。相比之下，无毒力菌株以及能够定殖于派伊尔结的无毒力菌株均不会增加凋亡细胞的背景水平。体内研究显示巨噬细胞、T细胞和B细胞都会发生凋亡。这些结果表明，细胞凋亡可能在志贺菌病的发病机制中起关键作用。
