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使用快速³H-葡萄糖掺入微量测定法鉴定白色念珠菌的两性霉素B耐药菌株。

Identification of an amphotericin B resistant strain of Candida albicans using a rapid 3H-glucose incorporation microassay.

作者信息

Sweeney J F, Greene J N, Hiemenz J W, Wei S, Rosemurgy A S, Djeu J Y

机构信息

Department of Surgery, University of South Florida, College of Medicine, Tampa, USA.

出版信息

J Infect. 1996 Nov;33(3):221-6. doi: 10.1016/s0163-4453(96)92369-5.

DOI:10.1016/s0163-4453(96)92369-5
PMID:8945714
Abstract

Using a 3H-glucose incorporation assay, antifungal sensitivity testing undertaken on an isolate of Candida albicans cultured from the blood of a bone marrow transplant patient documented resistance to amphotericin B but sensitivity to fluconazole and itraconazole. Information obtained from in vitro antifungal sensitivity testing can be used to direct in vivo antifungal therapy. Widespread application of standardized in vitro antifungal sensitivity testing is needed.

摘要

通过3H-葡萄糖掺入试验,对一名骨髓移植患者血液中培养的白色念珠菌分离株进行抗真菌敏感性测试,结果显示该菌株对两性霉素B耐药,但对氟康唑和伊曲康唑敏感。体外抗真菌敏感性测试获得的信息可用于指导体内抗真菌治疗。需要广泛应用标准化的体外抗真菌敏感性测试。

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