Ro L S, Chen S T, Tang L M, Chang H S
Department of Neurology, Chang Gung Memorial Hospital, Taipei, Taiwan, ROC.
Neurosci Lett. 1996 Nov 1;218(2):87-90. doi: 10.1016/s0304-3940(96)13109-8.
Systemic administration of anti-nerve growth factor (NGF) antibodies can block nociceptive fiber sprouting into denervated adult rat skin. However, the effect of local application of anti-NGF on collateral sprouting in rats caused by chronic constriction injury (CCI) has not been well studied. We investigated the influence of local anti-NGF on collateral sprouting caused by CCI of the rat's sciatic nerve. Using a dye-labeled plasma extravasation technique, we can easily evaluate the extent of collateral sprouting by the clearly defined blue coloration area. Our results showed that local application of anti-NGF either in a high or low dose significantly prevented the spread of collateral sprouting from the saphenous nerve into the sciatic innervation territory. In contrast, distilled water did not show a significant block of the saphenous nerve collateral sprouting. Our study suggests that collateral sprouting is dependent on the local availability of NGF by the nearby intact cutaneous nerve fibers.
全身给予抗神经生长因子（NGF）抗体可阻断伤害性纤维向去神经支配的成年大鼠皮肤内的芽生。然而，局部应用抗NGF对大鼠慢性压迫性损伤（CCI）所致侧支芽生的影响尚未得到充分研究。我们研究了局部抗NGF对大鼠坐骨神经CCI引起的侧支芽生的影响。使用染料标记的血浆外渗技术，我们可以通过清晰界定的蓝色染色区域轻松评估侧支芽生的程度。我们的结果表明，高剂量或低剂量局部应用抗NGF均能显著阻止隐神经侧支芽生扩散至坐骨神经支配区域。相比之下蒸馏水对隐神经侧支芽生没有显著的阻断作用。我们的研究表明，侧支芽生依赖于附近完整皮肤神经纤维局部可获得的NGF。
