小鼠隐静脉穿刺和改良尾静脉采血的评估
Evaluation of saphenous venipuncture and modified tail-clip blood collection in mice.
作者信息
Abatan Omorodola I, Welch Kathleen B, Nemzek Jean A
机构信息
Unit for Laboratory Animal Medicine, University of Michigan, Ann Arbor, MI, USA.
出版信息
J Am Assoc Lab Anim Sci. 2008 May;47(3):8-15.
The purpose of this study was to evaluate the effects of 2 methods of blood collection in unanesthetized mice. The saphenous venipuncture method was compared with a modified tail-clip technique that requires minimal restraint. Mice were evaluated through behavioral observation and plasma corticosterone levels. The results showed that the 2 methods produced similar corticosterone responses and that the tail-clip method produced fewer behavioral reactions. In addition, the effects of saphenous venipuncture method appeared to be dependent on the handler's technical expertise. When a series of 4 blood collections were performed over 1 wk, the 2 methods yielded similar corticosterone levels that did not increase over time. Some of the behavioral signs appeared to increase over the series of blood collections obtained by the saphenous venipuncture method. Serial complete blood counts showed that the tail vessels yielded higher total white blood cell, neutrophil, and lymphocyte counts than did the saphenous vein. Neither method appeared to cause stress-associated changes in the leukogram after serial blood collection. Overall, the effects of modified tail-clip method were similar to those of the saphenous venipuncture method in unanesthetized mice.
本研究的目的是评估两种采血方法对未麻醉小鼠的影响。将隐静脉穿刺法与一种需极少约束的改良尾夹技术进行比较。通过行为观察和血浆皮质酮水平对小鼠进行评估。结果显示，两种方法产生的皮质酮反应相似，且尾夹法产生的行为反应较少。此外，隐静脉穿刺法的效果似乎取决于操作者的技术专长。当在1周内进行一系列4次采血时，两种方法产生的皮质酮水平相似，且不会随时间增加。通过隐静脉穿刺法进行的一系列采血过程中，一些行为体征似乎有所增加。连续全血细胞计数显示，尾血管产生的总白细胞、中性粒细胞和淋巴细胞计数高于隐静脉。两种方法在连续采血后似乎均未引起与应激相关的白细胞计数变化。总体而言，在未麻醉小鼠中，改良尾夹法的效果与隐静脉穿刺法相似。
Zotero 插件