Purnelle B, Goffeau A
Unité de Biochimie Physiologíque, Université Catholique de Louvain, Louvain-la-Neuve, Belgium.
Yeast. 1996 Nov;12(14):1475-81. doi: 10.1002/(sici)1097-0061(199611)12:14<1475::aid-yea32>3.0.co;2-v.
We have determined the nucleotide sequence of a 40 kb fragment from the right arm of chromosome XV of Saccharomyces cerevisiae. Subsequent analysis revealed 18 non-overlapping open reading frames (ORFs) numbered from 06257 to 06357, an ARS, two tRNA genes and a Ty2 with its flanking elements. Ten ORFs have been sequenced previously: TEA1, RPA43, RPA190, SGC1 (also called TYE7) REV1, PUT4, CIN1, MNE and MRE4 (also called MEK1). Among the others, two seem to code for a new pyruvate kinase and for a new ubiquitin-conjugating enzyme; three have interesting homology with genes located on the left arm of chromosome I. This similarity with chromosome I extends to the left of the sequence presented here (Parle et al., submitted to Yeast). The homologous genes on the two chromosomes are placed in the same relative order.
我们已经确定了酿酒酵母第十五号染色体右臂上一个40 kb片段的核苷酸序列。随后的分析揭示了18个不重叠的开放阅读框（ORF），编号从06257到06357，一个自主复制序列（ARS），两个tRNA基因以及一个带有侧翼元件的Ty2。之前已经对10个ORF进行了测序：TEA1、RPA43、RPA190、SGC1（也称为TYE7）、REV1、PUT4、CIN1、MNE和MRE4（也称为MEK1）。在其他的ORF中，有两个似乎编码一种新的丙酮酸激酶和一种新的泛素结合酶；有三个与位于第一号染色体左臂上的基因具有有趣的同源性。这种与第一号染色体的相似性延伸到了此处呈现序列的左侧（Parle等人，已提交给《酵母》杂志）。两条染色体上的同源基因以相同的相对顺序排列。
