Eliakim R, Alpers D H, Oren R, Fich A, DeSchryver-Kecskemeti K
Division of Gastroenterology, Washington University School of Medicine, St Louis, Missouri 63110, USA.
Gut. 1996 Sep;39(3):353-9. doi: 10.1136/gut.39.3.353.
Surfactant-like particles, normal products of the human enterocyte, are released into the lumen and secreted into blood.
To assess their role as markers for mucosal functional integrity, this study examined their content in biopsy specimens and serum of patients with duodenal ulcer disease, compared with non-diseased control subjects.
Endoscopic biopsy specimens were taken 1-2 cm from areas of active inflammation or ulcer (peptic ulcer patients) or just beyond the duodenal bulb (normals) in 35 consecutive subjects.
After staining for phospholipid, extracellular and intracellular particles were counted on transmission electron micrographs of coded specimens. Serum was obtained from 24 patients, and densitometry of the 59 kDa band detected on western blot by antiserum against human jejunal particle was measured.
Normal duodenum (n = 15) contained more particles (44 (4.7)) particles/block, mean (SD) than active duodenal ulcer (n = 13, 17 (3.9)) or gastritis/duodenitis patients (n = 4, 9 (2.7)). Three patients examined after healing of duodenal ulcers showed abundant particles (n = 3, 67 (2.2)). Similarly, the 59 kDa band was decreased in serum of patients with active peptic ulcer disease (n = 11, 0.25 (0.04) absorbance units) compared with normal patients (n = 10, 0.40 (0.03)) or healed ulcers (n = 3, 0.62 (0.04)). There was good correlation between morphological mucosal particle abundance and particle protein content of serum assayed from the same patients (r = 0.831). These changes were independent of Helicobacter pylori status.
The mucosal and serum content of surfactant-like particles may reflect general mucosal integrity of the enterocytes from which they are derived.
表面活性剂样颗粒是人类肠上皮细胞的正常产物，会释放到肠腔并分泌入血。
为评估其作为黏膜功能完整性标志物的作用，本研究检测了十二指肠溃疡病患者活检标本和血清中的表面活性剂样颗粒含量，并与无病对照者进行比较。
连续选取35名受试者，在内镜检查时，从距活动性炎症或溃疡部位（消化性溃疡患者）1 - 2厘米处或十二指肠球部以外（正常人）取材，获取内镜活检标本。
对编码标本的透射电子显微镜图像进行磷脂染色后，计数细胞外和细胞内颗粒。从24名患者获取血清，检测抗人空肠颗粒抗血清在蛋白质免疫印迹法上检测到的59 kDa条带的光密度。
正常十二指肠（n = 15）每块标本含有的颗粒（44（4.7））颗粒数，平均值（标准差）多于活动性十二指肠溃疡（n = 13，17（3.9））或胃炎/十二指肠炎患者（n = 4，9（2.7））。3名十二指肠溃疡愈合后的患者检测显示颗粒丰富（n = 3，67（2.2））。同样，与正常患者（n = 10，0.40（0.03））或溃疡愈合患者（n = 3，0.62（0.04））相比，活动性消化性溃疡病患者（n = 11，0.25（0.04）吸光度单位）血清中的59 kDa条带降低。同一患者的形态学黏膜颗粒丰度与血清颗粒蛋白含量之间存在良好相关性（r = 0.831）。这些变化与幽门螺杆菌感染状态无关。
表面活性剂样颗粒的黏膜和血清含量可能反映其来源肠上皮细胞的总体黏膜完整性。
