Mano H, Roa I, Araya J C, Ohta T, Yoshida K, Araki K, Kinebuchi H, Ishizu T, Nakadaira H, Endoh K, Yamamoto M, Watanabe H
Department of Hygiene and Preventive Medicine, Niigata University School of Medicine, Japan.
Mutat Res. 1996 Nov 4;371(1-2):73-7. doi: 10.1016/s0165-1218(96)90096-8.
The mutagenic activity of bile was compared between Chilean and Japanese female patients having cholelithiasis by the Ames assay using Salmonella typhimurium tester strain TA98 in the presence of S9 mix with blue rayon adsorption technique. A reason for conducting the present investigation is that Chile and Japan have the highest mortality rates for the gallbladder cancer (GBC) in the world. Of 24 bile samples collected in Chile, 20 (83.3%) samples showed mutagenicity. In the case of Japanese bile, 21 (80.8%) of 26 and 5 (19.2%) of 26 cases were mutagenic in samples from high- and low-risk areas for GBC, respectively. Therefore, both the Chilean and the Japanese samples collected in high-risk areas showed higher mutagenic rates than the Japanese ones in a low-risk area, with a statistical significance (p < 0.001), chi-square test). The average number of revertant colonies were 128 +/- 92 (mean +/- SD), 62 +/- 14 and 66 +/- 13, respectively, when the blue rayon extracts of 200 microliters bile were applied to the Ames test. Thus, Chilean bile had a tendency to show a higher mutagenic activity than Japanese.
采用鼠伤寒沙门氏菌测试菌株TA98，在存在S9混合液的情况下，运用蓝色人造丝吸附技术，通过艾姆斯试验比较了智利和日本患有胆结石的女性患者胆汁的致突变活性。开展本研究的一个原因是智利和日本是世界上胆囊癌（GBC）死亡率最高的国家。在智利收集的24份胆汁样本中，20份（83.3%）样本显示有致突变性。对于日本胆汁样本，在GBC高风险地区的26份样本中有21份（80.8%）有致突变性，在低风险地区的26份样本中有5份（19.2%）有致突变性。因此，在高风险地区收集的智利和日本样本的致突变率均高于日本低风险地区的样本，具有统计学意义（p < 0.001，卡方检验）。当将200微升胆汁的蓝色人造丝提取物用于艾姆斯试验时，回复菌落的平均数量分别为128±92（平均值±标准差）、62±14和66±13。因此，智利胆汁显示出比日本胆汁更高的致突变活性趋势。
