Hori Y, Nakamura K, Yamamoto M, Shimada K, Shibuya N, Yoshida K, Ogoshi K, Kinebuchi H, Araki K
Department of Hygiene and Preventive Medicine, Niigata University School of Medicine, Niigata, Japan.
Mutat Res. 1999 Jul 21;444(1):41-7. doi: 10.1016/s1383-5718(99)00071-6.
The concentrations and compositions of free fatty acids (FFAs) in human bile, especially of inhibitory free fatty acids (IFFAs), were analyzed in terms of anti-mutagenic effects in relation to the mutagenic activity of bile. Bile samples were collected from patients with cholelithiasis residing in either Niigata or Kochi prefectures of Japan, regions characterized as the highest and lowest risk areas for gallbladder cancer (GBC), respectively. Biliary FFAs and IFFAs were analyzed by high-performance liquid chromatography and mutagenicity was examined in by the Ames test (TA98+S9mix) after blue rayon treatment. There was a tendency for higher biliary FFA and IFFA concentrations in the Kochi subjects, but the proportion of IFFA to the total FFA concentration did not differ between the two areas. There was an inverse correlation between the concentrations of IFFAs and the numbers of revertant colonies in both Niigata and Kochi subjects. However, at a dose of 591 micromol/l, (calculated based on the average amount of IFFAs absorbed in blue rayon) IFFAs did not exhibit anti-mutagenic actions in the blue rayon extracts. Within this range, more positive samples were seen in Niigata than in Kochi, suggesting the presence of more active mutagen(s) in Niigata samples.
就胆汁的诱变活性而言，对人胆汁中游离脂肪酸（FFA）的浓度和组成，特别是抑制性游离脂肪酸（IFFA）的浓度和组成进行了抗诱变作用分析。胆汁样本取自居住在日本新潟县或高知县的胆结石患者，这两个地区分别被视为胆囊癌（GBC）风险最高和最低的地区。通过高效液相色谱法分析胆汁中的FFA和IFFA，并在经蓝人造丝处理后，采用艾姆斯试验（TA98+S9混合物）检测其诱变性。高知县患者的胆汁FFA和IFFA浓度有升高趋势，但两个地区的IFFA占总FFA浓度的比例并无差异。新潟县和高知县患者的IFFA浓度与回复菌落数均呈负相关。然而，在591微摩尔/升的剂量下（根据蓝人造丝吸收的IFFA平均量计算），IFFA在蓝人造丝提取物中未表现出抗诱变作用。在此范围内，新潟县的阳性样本比高知县更多，这表明新潟县样本中存在更多活性诱变剂。
