Neuman-Silberberg F S, Schüpbach T
HHMI, Department of Molecular Biology, Princeton University, NJ 08544, USA.
Mech Dev. 1996 Oct;59(2):105-13. doi: 10.1016/0925-4773(96)00567-9.
The establishment of anterior-posterior and dorsal-ventral polarity of the Drosophila egg and embryo depends on the function of the genes gurken, cornichon and Egfr (Drosophila epidermal growth factor receptor homolog). These genes encode components of a signal transduction pathway that transmits information between the germline cells and the somatic follicle cells of the ovary. gurken encodes a transforming growth factor-alpha-like protein and is a putative germline ligand of the Egfr present on the follicle cells. In mid-oogenesis the gurken transcript becomes spatially localized to the future dorsal-anterior cortex of the oocyte. To analyze the distribution pattern of Gurken protein we prepared antibodies against Gurken. We describe here the distribution pattern of the Gurken protein in wild-type ovaries and in ovaries from a number of dorsal-ventral patterning mutants. By immunoblotting we detect one major form of the Gurken protein, which likely corresponds to the unprocessed protein.
果蝇卵和胚胎前后极性与背腹极性的建立取决于基因gurken、cornichon和Egfr（果蝇表皮生长因子受体同源物）的功能。这些基因编码一种信号转导途径的组成部分，该途径在卵巢的生殖细胞和体细胞滤泡细胞之间传递信息。gurken编码一种转化生长因子-α样蛋白，是滤泡细胞上Egfr的一种假定生殖系配体。在卵子发生中期，gurken转录本在空间上定位到卵母细胞未来的背前部皮质。为了分析Gurken蛋白的分布模式，我们制备了针对Gurken的抗体。我们在此描述了Gurken蛋白在野生型卵巢和一些背腹模式突变体卵巢中的分布模式。通过免疫印迹，我们检测到Gurken蛋白的一种主要形式，它可能对应于未加工的蛋白。
