Polotsky Y E, Dragunskaya E M, Samostrelsky A Y, Vasser N R, Efremov V E, Snigirevskaya E S, Seliverstova V G
Med Biol. 1977 Jun;55(3):130-40.
Light and electron microscope studies in ligated rabbit gut loops showed that cholera vibrios El Tor attached themselves to the enterocytes and multiplied, most probably on the intestinal epithelium surface. But they did not penetrate deeper and were unable to cause destruction of the epithelium or marked inflammation. Ultrastructural manifestations of both apocrine and merocrine secretion of the enterocytes were observed. In addition, the enterocytes showed signs of generally enhanced activity. Some other changes and alterations in the lamina propria, presumably induced by excessive transport of water, are also described. The enterotoxin-induced epithelial hypersecretion with fluid accumulation in the gut loops resulted in the attached vibrios being cleared off the epithelium lining into the gut lumen. NAG vibrios were mostly incapable of inducing the above changes. We discuss the importance of cholera vibrio attachment to and its multiplication on the intestinal epithelium in the pathogenesis of cholera.
对结扎的兔肠袢进行光镜和电镜研究表明，埃尔托霍乱弧菌附着于肠上皮细胞并繁殖，很可能是在肠上皮表面。但它们并未深入穿透，也无法导致上皮细胞破坏或明显炎症。观察到肠上皮细胞顶浆分泌和局部分泌的超微结构表现。此外，肠上皮细胞显示出总体活性增强的迹象。还描述了固有层中的一些其他变化，推测是由过度的水转运引起的。肠毒素诱导的上皮细胞高分泌以及肠袢中液体蓄积导致附着的弧菌从肠上皮衬里清除到肠腔中。非产毒株霍乱弧菌大多无法诱导上述变化。我们讨论了霍乱弧菌在肠道上皮的附着及其繁殖在霍乱发病机制中的重要性。
