Davis P J, Wiese D R, Rosazza J P
Lloydia. 1977 May-Jun;40(3):239-46.
Microbial transformation experiments have been conducted with the antitumor alkaloid, d-tetrandrine. The alkaloid is selectively demethylated at the N-(2)-position by Cunninghamella blakesleeana ATCC 8688a. The biotransformation reaction is highly selective and uncomplicated by side-product formation. N-demethylation of the alkaloid using methylchloroformate is not regio-specific. Procedures used in screening microorganisms for their abilities to yield metabolites of d-tetrandrine are evaluated.
已使用抗肿瘤生物碱汉防己甲素进行了微生物转化实验。该生物碱被布氏小克银汉霉ATCC 8688a在N-(2)位选择性去甲基化。生物转化反应具有高度选择性，且不会因副产物形成而变得复杂。使用氯甲酸甲酯对该生物碱进行N-去甲基化反应不具有区域特异性。对用于筛选微生物产生汉防己甲素代谢物能力的方法进行了评估。
