Barrientos A, Casademont J, Rötig A, Miró O, Urbano-Márquez A, Rustin P, Cardellach F
Departament of Internal Medicine, Hospital Clínic i Provincial, Universitat de Barcelona, Spain.
Biochem Biophys Res Commun. 1996 Dec 13;229(2):536-9. doi: 10.1006/bbrc.1996.1839.
We investigated the relationship between age and respiratory chain function of skeletal muscle mitochondria in 132 control individuals (15 to 95 years old). Muscle mitochondria were studied polarographically and spectrophotometrically. By regression analysis, we found a significant inverse correlation between age and oxygen uptake linked to substrate oxidation (succinate, glutamate-malate or ascorbate-TMPD). However, this significance disappeared after including physical activity and tobacco consumption as confounding variables in a multivariate statistical analysis. Similarly, the activity of respiratory chain complexes individually measured did not decline with age. It therefore appears that respiratory chain activity in human skeletal muscle mitochondria is substantially undamaged during the aging process.
我们研究了132名对照个体（年龄在15至95岁之间）的年龄与骨骼肌线粒体呼吸链功能之间的关系。采用极谱法和分光光度法对肌肉线粒体进行了研究。通过回归分析，我们发现与底物氧化（琥珀酸、谷氨酸-苹果酸或抗坏血酸-TMPD）相关的氧气摄取量与年龄之间存在显著的负相关。然而，在多变量统计分析中将身体活动和烟草消费作为混杂变量纳入后，这种显著性消失了。同样，单独测量的呼吸链复合物的活性也不会随年龄下降。因此，在衰老过程中，人类骨骼肌线粒体中的呼吸链活性似乎基本未受损。
