Suzuki H, Kawai J, Taga C, Yaoi T, Hara A, Hirose K, Hayashizaki Y, Watanabe S
Shionogi Institute for Medical Science, Mishima 2-5-1, Settsu-shi, Osaka, 566, Japan.
Biochem Biophys Res Commun. 1996 Dec 24;229(3):902-9. doi: 10.1006/bbrc.1996.1900.
In RLGS-M (restriction landmark genomic scanning using methylation-sensitive endonuclease) displays of mouse brains, spot #91 is one of tissue-specific gel spots whose intensity changes developmentally. We have now cloned the corresponding cDNA from this spot and analyzed its structure and expression. The deduced amino acid sequence revealed that the #91 cDNA encodes a novel protein of 403 amino acids which consists of a cysteine-rich domain and a SH3 domain. We designated this gene as Stac. Northern blotting and in situ hybridization analyses demonstrated that 2.7 kb of Stac mRNA is expressed predominantly in brain and neurons, especially in hippocampus, cerebellum and inferior olive. Further, the gene product of 47 kDa was found by western blotting analysis in the soluble fractions of brain as well as Stac-expression vector-transfected NIH3T3 cells. Although the function of Stac is unknown so far, it is likely involved in a neuron-specific signal transduction.
在小鼠大脑的RLGS-M（使用甲基化敏感内切核酸酶的限制性地标基因组扫描）显示中，斑点#91是组织特异性凝胶斑点之一，其强度随发育而变化。我们现已从该斑点克隆出相应的cDNA，并分析了其结构和表达。推导的氨基酸序列显示，#91 cDNA编码一种由403个氨基酸组成的新蛋白质，该蛋白质由富含半胱氨酸的结构域和SH3结构域组成。我们将该基因命名为Stac。Northern印迹和原位杂交分析表明，2.7 kb的Stac mRNA主要在脑和神经元中表达，尤其是在海马体、小脑和下橄榄核中。此外，通过蛋白质印迹分析在脑的可溶性部分以及转染了Stac表达载体的NIH3T3细胞中发现了47 kDa的基因产物。尽管目前Stac的功能尚不清楚，但它可能参与神经元特异性信号转导。
