Sankalé J L, De La Tour R S, Marlink R G, Scheib R, Mboup S, Essex M E, Kanki P J
Harvard AIDS Institute, Harvard School of Public Health, Boston, Massachusetts 02115, USA.
Virology. 1996 Dec 15;226(2):418-23. doi: 10.1006/viro.1996.0671.
Sequences of the HIV-2 envelope C2-C3 region were obtained after direct PCR amplification of proviral DNA from the brain and peripheral blood mononuclear cells of an HIV-2-infected AIDS patient. Tissue-specific sequences confirmed previous observations that HIV-2 is indeed present in the central nervous system of infected individuals. Distinct but related quasi-species were present in these two different tissues of the same individual. Residues at position 18 and 19 of the V3 loop and overall charge of the loop suggest that the brain virus was NSI/macrophage tropic; whereas the sequences from the two blood samples were indicative of a SI/T-cell tropic virus. This is the first description of these two genotypes in the same HIV-2-infected individual. Analysis of more samples from different compartments would help to better understand tissue-specific factors in quasi-species evolution in vivo.
从一名感染HIV-2的艾滋病患者的大脑和外周血单核细胞中提取前病毒DNA，经直接PCR扩增后获得了HIV-2包膜C2-C3区域的序列。组织特异性序列证实了先前的观察结果，即HIV-2确实存在于受感染个体的中枢神经系统中。在同一个体的这两种不同组织中存在不同但相关的准种。V3环第18和19位的残基以及环的总电荷表明，脑内病毒具有非SI/巨噬细胞嗜性；而两份血液样本的序列表明是SI/T细胞嗜性病毒。这是首次在同一感染HIV-2的个体中描述这两种基因型。分析来自不同区室的更多样本将有助于更好地理解体内准种进化中的组织特异性因素。
