Gentner N E
Mol Gen Genet. 1977 Jul 20;154(2):129-33. doi: 10.1007/BF00330828.
The major part of the substantial gamma-resistance of wild-type Schizosaccharomyces pombe appears to be due to prereplicative recombinational repair mechanisms. The existence of a second "prereplicative G2" repair pathway, specific for gamma-induced damage, has now been deduced from studies of the effect of the repair inhibitor caffeine on gamma-irradiated G1 phase and G2 phase cells. only G2 cells are additionally inactivated on exposure to caffeine after gamma-irradiation. This shows that both known caffeine-sensitive gamma-repair processes (Gentner and wener, Molec. gen. Genet. 145, 1-5 [1976]) are dependent on the presence of a duplicated genome (2c) at the time of radiation exposure. Pathway I is the known "prereplicative G2" repair process (Fabre, Radiation Res. 56, 528-539 [1973]) which is involved in both UV- and gamma-repair, and which requires post-irradiation protein synthesis for activity. Pathway II represents a second distinct "prereplicative G2" repair mechanism; it differs from the first in that it is specific for repair of gamma-induced damage and appears to be constitutive.
野生型粟酒裂殖酵母对γ射线具有较强抗性，其主要原因似乎是复制前的重组修复机制。通过研究修复抑制剂咖啡因对γ射线照射的G1期和G2期细胞的影响，现已推断出存在第二种“复制前G2期”修复途径，该途径对γ射线诱导的损伤具有特异性。只有G2期细胞在γ射线照射后暴露于咖啡因时会额外失活。这表明，已知的两种对咖啡因敏感的γ射线修复过程（Gentner和Wener，《分子遗传学与普通遗传学》145卷，1 - 5页[1976年]）都依赖于辐射暴露时双倍体基因组（2c）的存在。途径I是已知的“复制前G2期”修复过程（Fabre，《辐射研究》56卷，528 - 539页[1973年]），它参与紫外线和γ射线修复，并且其活性需要辐射后蛋白质合成。途径II代表第二种不同的“复制前G2期”修复机制；它与第一种机制的不同之处在于，它对γ射线诱导的损伤修复具有特异性，并且似乎是组成型的。
