Cribier B, Rey D, Uhl G, Schmitt C, Libbrecht E, Vetter D, Lang J M, Kirn A, Stoll-Keller F
INSERM U74, Strasbourg, France.
Res Virol. 1996 Nov-Dec;147(6):325-32. doi: 10.1016/s0923-2516(97)85124-2.
In patients chronically infected by hepatitis C virus (HCV), peripheral blood mononuclear cells (PBMCs) were shown to be targets for virus replication and in those coinfected with HIV, HCV viraemia was considerably increased. The purpose of this study was to quantify HCV RNA in PBMCs from 25 patients infected by HCV and from 25 patients coinfected by HCV and HIV. We used the branched DNA assay after extraction of total RNA on 5 x 10(6) cells to quantify HCV RNA, and the Inno LiPA assay to determine the HCV genotype. HCV RNA in PBMCs could be quantified in 8/25 patients in each group, but the HCV RNA concentration was very low in comparison with viraemia, since the highest result was 8.1 x 10(4) Eq genome/10(6) cells. In 10 ml of total blood, there was approximately 100 to 5,000 times less HCV RNA in PBMCs than in the plasma. It is therefore likely that PBMCs play only a minor part in the viral load present in the plasma. There was no preferential genotype associated with quantifiable HCV RNA in the PBMCs. In the case of HIV coinfection, there was no increase in the HCV-RNA concentration in PBMCs that could explain the increased viraemia observed in these patients. On the contrary, HCV RNA could not even be detected by RT-PCR in some of our coinfected patients.
在慢性丙型肝炎病毒（HCV）感染患者中，外周血单个核细胞（PBMCs）被证明是病毒复制的靶细胞，而在合并感染HIV的患者中，HCV病毒血症显著增加。本研究的目的是对25例HCV感染患者和25例HCV与HIV合并感染患者的PBMCs中的HCV RNA进行定量。我们在从5×10⁶个细胞中提取总RNA后，使用分支DNA分析法对HCV RNA进行定量，并使用Inno LiPA分析法确定HCV基因型。每组25例患者中，有8例患者的PBMCs中的HCV RNA能够被定量，但与病毒血症相比，HCV RNA浓度非常低，因为最高结果为8.1×10⁴ Eq基因组/10⁶个细胞。在10 ml全血中，PBMCs中的HCV RNA比血浆中的少约100至5000倍。因此，PBMCs在血浆中的病毒载量中可能仅起次要作用。PBMCs中可定量的HCV RNA不存在与之相关的优势基因型。在合并感染HIV的情况下，PBMCs中的HCV-RNA浓度没有增加，无法解释这些患者中观察到的病毒血症增加。相反，在我们的一些合并感染患者中，甚至通过RT-PCR也检测不到HCV RNA。
