Michaelis O E, Szepesi B
Nutr Metab. 1977;21(6):329-40. doi: 10.1159/000176081.
Male rats were starved and refed diets containing 40% disaccharides (maltose, trehalose, sucrose, turanose), trisaccharide (melezitose), starch, or the monosaccharide equivalents. Responses of hepatic glucose-6-phosphate dehydrogenase (G6PD), malic enzyme (ME), fatty acid synthetase (FAS), and total liver lipid (TLL) or changes in concentration of portal blood total carbohydrate and fructose were determined following refeeding of the different carbohydrate diets. Maltose, trehalose, sucrose, and turanose refeeding resulted in G6PD and ME responses which were higher than the responses to their component monosaccharides (disaccharide effect). Starch refeeding decreased the responses of G6PD, ME, and FAS when compared to refeeding of glucose. Refeeding diets containing fructose (sucrose, turanose, melezitose, and monosaccharide equivalents containing fructose) increased the responses of G6PD, ME, FAS, and TLL. No correlation between portal blood carbohydrate concentration and hepatic enzyme levels could be demonstrated. It is concluded that readily digestible disaccharides produce an effect which is greater than the effect produced by their monosaccharide equivalents. If these disaccharide configurations are fed as part of a trisaccharide or polysaccharide, the disaccharide effect is no longer discernible.
将雄性大鼠饥饿后再喂食含40%二糖（麦芽糖、海藻糖、蔗糖、松二糖）、三糖（蜜三糖）、淀粉或单糖等价物的日粮。在喂食不同碳水化合物日粮后，测定肝脏葡萄糖-6-磷酸脱氢酶（G6PD）、苹果酸酶（ME）、脂肪酸合成酶（FAS）以及肝脏总脂质（TLL）的反应，或门静脉血总碳水化合物和果糖浓度的变化。重新喂食麦芽糖、海藻糖、蔗糖和松二糖后，G6PD和ME的反应高于对其组成单糖的反应（二糖效应）。与喂食葡萄糖相比，重新喂食淀粉会降低G6PD、ME和FAS的反应。重新喂食含果糖的日粮（蔗糖、松二糖、蜜三糖以及含果糖的单糖等价物）会增加G6PD、ME、FAS和TLL的反应。门静脉血碳水化合物浓度与肝脏酶水平之间未显示出相关性。得出的结论是，易消化的二糖产生的效应大于其单糖等价物产生的效应。如果将这些二糖构型作为三糖或多糖的一部分喂食，则不再能看出二糖效应。
